This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gibson, J.
Right arrow Articles by Harwood, C. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gibson, J.
Right arrow Articles by Harwood, C. S.

 Previous Article  |  Next Article 

J. Bacteriol., Feb 1997, 634-642, Vol 179, No. 3
Copyright © 1997, American Society for Microbiology

4-hydroxybenzoyl coenzyme A reductase (dehydroxylating) is required for anaerobic degradation of 4-hydroxybenzoate by Rhodopseudomonas palustris and shares features with molybdenum-containing hydroxylases

J Gibson, M Dispensa and CS Harwood
Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA.

The anaerobic degradation of 4-hydroxybenzoate is initiated by the formation of 4-hydroxybenzoyl coenzyme A, with the next step proposed to be a dehydroxylation to benzoyl coenzyme A, the starting compound for a central pathway of aromatic compound ring reduction and cleavage. Three open reading frames, divergently transcribed from the 4- hydroxybenzoate coenzyme A ligase gene, hbaA, were identified and sequenced from the phototrophic bacterium Rhodopseudomonas palustris. These genes, named hbaBCD, specify polypeptides of 17.5, 82.6, and 34.5 kDa, respectively. The deduced amino acid sequences show considerable similarities to a group of hydroxylating enzymes involved in CO, xanthine, and nicotine metabolism that have conserved binding sites for [2Fe-2S] clusters and a molybdenum cofactor. Cassette disruption of the hbaB gene yielded a mutant that was unable to grow anaerobically on 4- hydroxybenzoate but grew normally on benzoate. The hbaB mutant cells did not accumulate [14C]benzoyl coenzyme A during short-term uptake of [14C]4-hydroxybenzoate, but benzoyl coenzyme A was the major radioactive metabolite formed by the wild type. In addition, crude extracts of the mutant failed to convert 4-hydroxybenzoyl coenzyme A to benzoyl coenzyme A. This evidence indicates that the hbaBCD genes encode the subunits of a 4-hydroxybenzoyl coenzyme A reductase (dehydroxylating). The sizes of the specified polypeptides are similar to those reported for 4-hydroxybenzoyl coenzyme A reductase isolated from the denitrifying bacterium Thauera aromatica. The amino acid consensus sequence for a molybdenum cofactor binding site is in HbaC. This cofactor appears to be an essential component because anaerobic growth of R. palustris on 4-hydroxybenzoate, but not on benzoate, was retarded unless 0.1 microM molybdate was added to the medium. Neither tungstate nor vanadate replaced molybdate, and tungstate competitively inhibited growth stimulation by molybdate.


This article has been cited by other articles:

  • Carmona, M., Zamarro, M. T., Blazquez, B., Durante-Rodriguez, G., Juarez, J. F., Valderrama, J. A., Barragan, M. J. L., Garcia, J. L., Diaz, E. (2009). Anaerobic Catabolism of Aromatic Compounds: a Genetic and Genomic View. Microbiol. Mol. Biol. Rev. 73: 71-133 [Abstract] [Full Text]  
  • Mouttaki, H., Nanny, M. A., McInerney, M. J. (2009). Metabolism of Hydroxylated and Fluorinated Benzoates by Syntrophus aciditrophicus and Detection of a Fluorodiene Metabolite. Appl. Environ. Microbiol. 75: 998-1004 [Abstract] [Full Text]  
  • Pan, C., Oda, Y., Lankford, P. K., Zhang, B., Samatova, N. F., Pelletier, D. A., Harwood, C. S., Hettich, R. L. (2008). Characterization of Anaerobic Catabolism of p-Coumarate in Rhodopseudomonas palustris by Integrating Transcriptomics and Quantitative Proteomics. Mol. Cell. Proteomics 7: 938-948 [Abstract] [Full Text]  
  • Chakraborty, R., Coates, J. D. (2005). Hydroxylation and Carboxylation--Two Crucial Steps of Anaerobic Benzene Degradation by Dechloromonas Strain RCB. Appl. Environ. Microbiol. 71: 5427-5432 [Abstract] [Full Text]  
  • Schmeling, S., Narmandakh, A., Schmitt, O., Gad'on, N., Schuhle, K., Fuchs, G. (2004). Phenylphosphate Synthase: a New Phosphotransferase Catalyzing the First Step in Anaerobic Phenol Metabolism in Thauera aromatica. J. Bacteriol. 186: 8044-8057 [Abstract] [Full Text]  
  • Schuhle, K., Fuchs, G. (2004). Phenylphosphate Carboxylase: a New C-C Lyase Involved in Anaerobic Phenol Metabolism in Thauera aromatica. J. Bacteriol. 186: 4556-4567 [Abstract] [Full Text]  
  • Rhee, S.-K., Liu, X., Wu, L., Chong, S. C., Wan, X., Zhou, J. (2004). Detection of Genes Involved in Biodegradation and Biotransformation in Microbial Communities by Using 50-Mer Oligonucleotide Microarrays. Appl. Environ. Microbiol. 70: 4303-4317 [Abstract] [Full Text]  
  • Boll, M., Fuchs, G., Meier, C., Trautwein, A., El Kasmi, A., Ragsdale, S. W., Buchanan, G., Lowe, D. J. (2001). Redox Centers of 4-Hydroxybenzoyl-CoA Reductase, a Member of the Xanthine Oxidase Family of Molybdenum-containing Enzymes. J. Biol. Chem. 276: 47853-47862 [Abstract] [Full Text]  
  • Schultz, A. C., Nygaard, P., Saxild, H. H. (2001). Functional Analysis of 14 Genes That Constitute the Purine Catabolic Pathway in Bacillus subtilis and Evidence for a Novel Regulon Controlled by the PucR Transcription Activator. J. Bacteriol. 183: 3293-3302 [Abstract] [Full Text]  
  • Egland, P. G., Harwood, C. S. (2000). HbaR, a 4-Hydroxybenzoate Sensor and FNR-CRP Superfamily Member, Regulates Anaerobic 4-Hydroxybenzoate Degradation by Rhodopseudomonas palustris. J. Bacteriol. 182: 100-106 [Abstract] [Full Text]  
  • Kang, B. S., Kim, Y. M. (1999). Cloning and Molecular Characterization of the Genes for Carbon Monoxide Dehydrogenase and Localization of Molybdopterin, Flavin Adenine Dinucleotide, and Iron-Sulfur Centers in the Enzyme of Hydrogenophaga pseudoflava. J. Bacteriol. 181: 5581-5590 [Abstract] [Full Text]  
  • Krooneman, J., van den Akker, S., Pedro Gomes, T. M., Forney, L. J., Gottschal, J. C. (1999). Degradation of 3-Chlorobenzoate under Low-Oxygen Conditions in Pure and Mixed Cultures of the Anoxygenic Photoheterotroph Rhodopseudomonas palustris DCP3 and an Aerobic Alcaligenes Species. Appl. Environ. Microbiol. 65: 131-137 [Abstract] [Full Text]  
  • Egland, P. G., Pelletier, D. A., Dispensa, M., Gibson, J., Harwood, C. S. (1997). A cluster of bacterial genes for anaerobic benzene ring biodegradation. Proc. Natl. Acad. Sci. USA 94: 6484-6489 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»