This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hill, T. M.
Right arrow Articles by Smith, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hill, T. M.
Right arrow Articles by Smith, J.

 Previous Article  |  Next Article 

J. Bacteriol., Mar 1997, 1931-1939, Vol 179, No. 6
Copyright © 1997, American Society for Microbiology

sfi-independent filamentation in Escherichia coli Is lexA dependent and requires DNA damage for induction

TM Hill, B Sharma, M Valjavec-Gratian and J Smith
Department of Microbiology and Immunology, University of North Dakota School of Medicine, Grand Forks 58202-9037, USA. tomhill@mail.med.und.nodak.edu

In Escherichia coli, damage to DNA induces the expression of a set of genes known collectively as the SOS response. Part of the SOS response includes genes that repair DNA damage, but another part of the response coordinates DNA replication and septation to prevent untimely cell division. The classic SOS gene product that inhibits cell division is SfiA (or SulA), which binds to FtsZ and prevents septum formation until the DNA damage has been repaired. However, another pathway acts to coordinate DNA replication and cell division when sfiA, or the sfi- dependent pathway, is inoperative. Until recently, little was known of this alternative pathway, which is called the sfi-independent pathway. We report here that sfi-independent filamentation is suppressed by lexA(Ind-) mutations, suggesting that derepression of the LexA regulon is necessary for sfi-independent induction. However, expression of LexA- controlled genes is not sufficient; DNA damage is also required to induce this secondary pathway of cell division inhibition. Furthermore, we postulate that loss of the common regulatory circuitry of the sfi- dependent and sfi-independent pathways by recA or lexA mutants uncouples cell division and DNA replication.


This article has been cited by other articles:

  • Aoki, S. K., Webb, J. S., Braaten, B. A., Low, D. A. (2009). Contact-Dependent Growth Inhibition Causes Reversible Metabolic Downregulation in Escherichia coli. J. Bacteriol. 191: 1777-1786 [Abstract] [Full Text]  
  • Fujimitsu, K., Su'etsugu, M., Yamaguchi, Y., Mazda, K., Fu, N., Kawakami, H., Katayama, T. (2008). Modes of Overinitiation, dnaA Gene Expression, and Inhibition of Cell Division in a Novel Cold-Sensitive hda Mutant of Escherichia coli. J. Bacteriol. 190: 5368-5381 [Abstract] [Full Text]  
  • Lau, S. Y., Zgurskaya, H. I. (2005). Cell Division Defects in Escherichia coli Deficient in the Multidrug Efflux Transporter AcrEF-TolC. J. Bacteriol. 187: 7815-7825 [Abstract] [Full Text]  
  • Rosenberger, C. M., Finlay, B. B. (2002). Macrophages Inhibit Salmonella Typhimurium Replication through MEK/ERK Kinase and Phagocyte NADPH Oxidase Activities. J. Biol. Chem. 277: 18753-18762 [Abstract] [Full Text]  
  • Sciochetti, S. A., Blakely, G. W., Piggot, P. J. (2001). Growth Phase Variation in Cell and Nucleoid Morphology in a Bacillus subtilis recA Mutant. J. Bacteriol. 183: 2963-2968 [Abstract] [Full Text]  
  • Raskin, D. M., de Boer, P. A. J. (1999). MinDE-Dependent Pole-to-Pole Oscillation of Division Inhibitor MinC in Escherichia coli. J. Bacteriol. 181: 6419-6424 [Abstract] [Full Text]  
  • Zahradka, D. (1999). Chromosome Segregation and Cell Division Defects in recBC sbcBC ruvC Mutants of Escherichia coli. J. Bacteriol. 181: 6179-6183 [Abstract] [Full Text]  
  • Elasri, M. O., Miller, R. V. (1999). Study of the Response of a Biofilm Bacterial Community to UV Radiation. Appl. Environ. Microbiol. 65: 2025-2031 [Abstract] [Full Text]  
  • Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]  
  • Draper, G. C., McLennan, N., Begg, K., Masters, M., Donachie, W. D. (1998). Only the N-Terminal Domain of FtsK Functions in Cell Division. J. Bacteriol. 180: 4621-4627 [Abstract] [Full Text]  
  • Smith, B. T., Walker, G. C. (1998). Mutagenesis and More: umuDC and the Escherichia coli SOS Response. Genetics 148: 1599-1610 [Abstract] [Full Text]  
  • Mukherjee, A., Cao, C., Lutkenhaus, J. (1998). Inhibition of FtsZ polymerization by SulA, an inhibitor of septation in Escherichia coli. Proc. Natl. Acad. Sci. USA 95: 2885-2890 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»