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J. Bacteriol., 03 1997, 2068-2072, Vol 179, No. 6
Copyright © 1997, American Society for Microbiology

Isolation and characterization of Rhizobium etli mutants altered in degradation of asparagine

A Huerta-Zepeda, L Ortuno, G Du Pont, S Duran, A Lloret, H Merchant-Larios and J Calderon
Departamento de Biotecnologia, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico D.F.

Rhizobium etli mutants unable to grow on asparagine as the nitrogen and carbon source were isolated. Two kinds of mutants were obtained: AHZ1, with very low levels of aspartase activity, and AHZ7, with low levels of asparaginase and very low levels of aspartase compared to the wild- type strain. R. etli had two asparaginases differentiated by their thermostabilities, electrophoretic mobilities, and modes of regulation. The AHZ mutants nodulated as did the wild-type strain and had nitrogenase levels similar to that of the wild-type strain.


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