This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Edgell, D. R.
Right arrow Articles by Doolittle, W. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Edgell, D. R.
Right arrow Articles by Doolittle, W. F.

 Previous Article  |  Next Article 

J. Bacteriol., Apr 1997, 2632-2640, Vol 179, No. 8
Copyright © 1997, American Society for Microbiology

Gene duplications in evolution of archaeal family B DNA polymerases

DR Edgell, HP Klenk and WF Doolittle
Department of Biochemistry, Dalhousie University, Halifax, Nova Scotia, Canada. dedgell@cs.dal.ca

All archaeal DNA-dependent DNA polymerases sequenced to date are homologous to family B DNA polymerases from eukaryotes and eubacteria. Presently, representatives of the euryarchaeote division of archaea appear to have a single family B DNA polymerase, whereas two crenarchaeotes, Pyrodictium occultum and Sulfolobus solfataricus, each possess two family B DNA polymerases. We have found the gene for yet a third family B DNA polymerase, designated B3, in the crenarchaeote S. solfataricus P2. The encoded protein is highly divergent at the amino acid level from the previously characterized family B polymerases in S. solfataricus P2 and contains a number of nonconserved amino acid substitutions in catalytic domains. We have cloned and sequenced the ortholog of this gene from the closely related Sulfolobus shibatae. It is also highly divergent from other archaeal family B DNA polymerases and, surprisingly, from the S. solfataricus B3 ortholog. Phylogenetic analysis using all available archaeal family B DNA polymerases suggests that the S. solfataricus P2 B3 and S. shibatae B3 paralogs are related to one of the two DNA polymerases of P. occultum. These sequences are members of a group which includes all euryarchaeote family B homologs, while the remaining crenarchaeote sequences form another distinct group. Archaeal family B DNA polymerases together constitute a monophyletic subfamily whose evolution has been characterized by a number of gene duplication events.


This article has been cited by other articles:

  • Sartori, A. A., Jiricny, J. (2003). Enzymology of Base Excision Repair in the Hyperthermophilic Archaeon Pyrobaculum aerophilum. J. Biol. Chem. 278: 24563-24576 [Abstract] [Full Text]  
  • Satomi, M., Oikawa, H., Yano, Y. (2003). Shewanella marinintestina sp. nov., Shewanella schlegeliana sp. nov. and Shewanella sairae sp. nov., novel eicosapentaenoic-acid-producing marine bacteria isolated from sea-animal intestines. Int. J. Syst. Evol. Microbiol. 53: 491-499 [Abstract] [Full Text]  
  • Fitz-Gibbon, S. T., Ladner, H., Kim, U.-J., Stetter, K. O., Simon, M. I., Miller, J. H. (2002). Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum. Proc. Natl. Acad. Sci. USA 10.1073/pnas.241636498v1 [Abstract] [Full Text]  
  • Gruz, P., Pisani, F. M., Shimizu, M., Yamada, M., Hayashi, I., Morikawa, K., Nohmi, T. (2001). Synthetic Activity of Sso DNA Polymerase Y1, an Archaeal DinB-like DNA Polymerase, Is Stimulated by Processivity Factors Proliferating Cell Nuclear Antigen and Replication Factor C. J. Biol. Chem. 276: 47394-47401 [Abstract] [Full Text]  
  • Barroso, G., Bois, F., Labarère, J. (2001). Duplication of a Truncated Paralog of the Family B DNA Polymerase Gene Aa-polB in the Agrocybe aegerita Mitochondrial Genome. Appl. Environ. Microbiol. 67: 1739-1743 [Abstract] [Full Text]  
  • Bohlke, K., Pisani, F. M., Vorgias, C. E., Frey, B., Sobek, H., Rossi, M., Antranikian, G. (2000). PCR performance of the B-type DNA polymerase from the thermophilic euryarchaeon Thermococcus aggregans improved by mutations in the Y-GG/A motif. Nucleic Acids Res 28: 3910-3917 [Abstract] [Full Text]  
  • Villarreal, L. P., DeFilippis, V. R. (2000). A Hypothesis for DNA Viruses as the Origin of Eukaryotic Replication Proteins. J. Virol. 74: 7079-7084 [Abstract] [Full Text]  
  • Kähler, M., Antranikian, G. (2000). Cloning and Characterization of a Family B DNA Polymerase from the Hyperthermophilic Crenarchaeon Pyrobaculum islandicum. J. Bacteriol. 182: 655-663 [Abstract] [Full Text]  
  • Komori, K., Ishino, Y. (2000). Functional interdependence of DNA polymerizing and 3'->5' exonucleolytic activities in Pyrococcus furiosus DNA polymerase I. Protein Eng Des Sel 13: 41-47 [Abstract] [Full Text]  
  • Cann, I. K. O., Ishino, S., Nomura, N., Sako, Y., Ishino, Y. (1999). Two Family B DNA Polymerases from Aeropyrum pernix, an Aerobic Hyperthermophilic Crenarchaeote. J. Bacteriol. 181: 5984-5992 [Abstract] [Full Text]  
  • Kelman, Z., Pietrokovski, S., Hurwitz, J. (1999). Isolation and Characterization of a Split B-type DNA Polymerase from the Archaeon Methanobacterium thermoautotrophicum Delta H. J. Biol. Chem. 274: 28751-28761 [Abstract] [Full Text]  
  • Schafer, G., Engelhard, M., Muller, V. (1999). Bioenergetics of the Archaea. Microbiol. Mol. Biol. Rev. 63: 570-620 [Abstract] [Full Text]  
  • Cann, I. K. O., Ishino, Y. (1999). Archaeal DNA Replication: Identifying the Pieces to Solve a Puzzle. Genetics 152: 1249-1267 [Abstract] [Full Text]  
  • Ishino, Y., Komori, K., Cann, I. K. O., Koga, Y. (1998). A Novel DNA Polymerase Family Found in Archaea. J. Bacteriol. 180: 2232-2236 [Abstract] [Full Text]  
  • Fitz-Gibbon, S. T., Ladner, H., Kim, U.-J., Stetter, K. O., Simon, M. I., Miller, J. H. (2002). Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum. Proc. Natl. Acad. Sci. USA 99: 984-989 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»