This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dinsmore, P. K.
Right arrow Articles by Klaenhammer, T. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dinsmore, P. K.
Right arrow Articles by Klaenhammer, T. R.

 Previous Article  |  Next Article 

J. Bacteriol., 05 1997, 2949-2957, Vol 179, No. 9
Copyright © 1997, American Society for Microbiology

Molecular characterization of a genomic region in a Lactococcus bacteriophage that is involved in its sensitivity to the phage defense mechanism AbiA

PK Dinsmore and TR Klaenhammer
Department of Food Science, North Carolina State University, Raleigh 27695, USA.

A spontaneous mutant of the lactococcal phage phi31 that is insensitive to the phage defense mechanism AbiA was characterized in an effort to identify the phage factor(s) involved in sensitivity of phi31 to AbiA. A point mutation was localized in the genome of the AbiA-insensitive phage (phi31A) by heteroduplex analysis of a 9-kb region. The mutation (G to T) was within a 738-bp open reading frame (ORF245) and resulted in an arginine-to-leucine change in the predicted amino acid sequence of the protein. The mutant phi31A-ORF245 reduced the sensitivity of phi31 to AbiA when present in trans, indicating that the mutation in ORF245 is responsible for the AbiA insensitivity of phi31A. Transcription of ORF245 occurs early in the phage infection cycles of phi31 and phi31A and is unaffected by AbiA. Expansion of the phi31 sequence revealed ORF169 (immediately upstream of ORF245) and ORF71 (which ends 84 bp upstream of ORF169). Two inverted repeats lie within the 84-bp region between ORF71 and ORF169. Sequence analysis of an independently isolated AbiA-insensitive phage, phi31B, identified a mutation (G to A) in one of the inverted repeats. A 118-bp fragment from phi31, encompassing the 84-bp region between ORF71 and ORF169, eliminates AbiA activity against phi31 when present in trans, establishing a relationship between AbiA and this fragment. The study of this region of phage phi31 has identified an open reading frame (ORF245) and a 118-bp DNA fragment that interact with AbiA and are likely to be involved in the sensitivity of this phage to AbiA.


This article has been cited by other articles:

  • Haaber, J., Rousseau, G. M., Hammer, K., Moineau, S. (2009). Identification and Characterization of the Phage Gene sav, Involved in Sensitivity to the Lactococcal Abortive Infection Mechanism AbiV. Appl. Environ. Microbiol. 75: 2484-2494 [Abstract] [Full Text]  
  • Haaber, J., Moineau, S., Fortier, L.-C., Hammer, K. (2008). AbiV, a Novel Antiphage Abortive Infection Mechanism on the Chromosome of Lactococcus lactis subsp. cremoris MG1363. Appl. Environ. Microbiol. 74: 6528-6537 [Abstract] [Full Text]  
  • Durmaz, E., Klaenhammer, T. R. (2007). Abortive Phage Resistance Mechanism AbiZ Speeds the Lysis Clock To Cause Premature Lysis of Phage-Infected Lactococcus lactis. J. Bacteriol. 189: 1417-1425 [Abstract] [Full Text]  
  • Bouchard, J. D., Moineau, S. (2004). Lactococcal Phage Genes Involved in Sensitivity to AbiK and Their Relation to Single-Strand Annealing Proteins. J. Bacteriol. 186: 3649-3652 [Abstract] [Full Text]  
  • Domingues, S., Chopin, A., Ehrlich, S. D., Chopin, M.-C. (2004). A Phage Protein Confers Resistance to the Lactococcal Abortive Infection Mechanism AbiP. J. Bacteriol. 186: 3278-3281 [Abstract] [Full Text]  
  • Sturino, J. M., Klaenhammer, T. R. (2004). Antisense RNA Targeting of Primase Interferes with Bacteriophage Replication in Streptococcus thermophilus. Appl. Environ. Microbiol. 70: 1735-1743 [Abstract] [Full Text]  
  • Domingues, S., Chopin, A., Ehrlich, S. D., Chopin, M.-C. (2004). The Lactococcal Abortive Phage Infection System AbiP Prevents both Phage DNA Replication and Temporal Transcription Switch. J. Bacteriol. 186: 713-721 [Abstract] [Full Text]  
  • Durmaz, E., Madsen, S. M., Israelsen, H., Klaenhammer, T. R. (2002). Lactococcus lactis Lytic Bacteriophages of the P335 Group Are Inhibited by Overexpression of a Truncated CI Repressor. J. Bacteriol. 184: 6532-6544 [Abstract] [Full Text]  
  • Bouchard, J. D., Dion, E., Bissonnette, F., Moineau, S. (2002). Characterization of the Two-Component Abortive Phage Infection Mechanism AbiT from Lactococcus lactis. J. Bacteriol. 184: 6325-6332 [Abstract] [Full Text]  
  • Sturino, J. M., Klaenhammer, T. R. (2002). Expression of Antisense RNA Targeted against Streptococcus thermophilus Bacteriophages. Appl. Environ. Microbiol. 68: 588-596 [Abstract] [Full Text]  
  • Madsen, S. M., Mills, D., Djordjevic, G., Israelsen, H., Klaenhammer, T. R. (2001). Analysis of the Genetic Switch and Replication Region of a P335-Type Bacteriophage with an Obligate Lytic Lifestyle on Lactococcus lactis. Appl. Environ. Microbiol. 67: 1128-1139 [Abstract] [Full Text]  
  • Kraus, J., Geller, B. L. (2001). Cloning of Genomic DNA of Lactococcus lactis That Restores Phage Sensitivity to an Unusual Bacteriophage sk1-Resistant Mutant. Appl. Environ. Microbiol. 67: 791-798 [Abstract] [Full Text]  
  • Walker, S. A., Klaenhammer, T. R. (2001). Leaky Lactococcus Cultures That Externalize Enzymes and Antigens Independently of Culture Lysis and Secretion and Export Pathways. Appl. Environ. Microbiol. 67: 251-259 [Abstract] [Full Text]  
  • Durmaz, E., Klaenhammer, T. R. (2000). Genetic Analysis of Chromosomal Regions of Lactococcus lactis Acquired by Recombinant Lytic Phages. Appl. Environ. Microbiol. 66: 895-903 [Abstract] [Full Text]  
  • Boucher, I., Émond, E., Dion, E., Montpetit, D., Moineau, S. (2000). Microbiological and molecular impacts of AbiK on the lytic cycle of Lactococcus lactis phages of the 936 and P335 species. Microbiology 146: 445-453 [Abstract] [Full Text]  
  • Walker, S. A., Klaenhammer, T. R. (2000). An Explosive Antisense RNA Strategy for Inhibition of a Lactococcal Bacteriophage. Appl. Environ. Microbiol. 66: 310-319 [Abstract] [Full Text]  
  • McGrath, S., Seegers, J. F. M. L., Fitzgerald, G. F., van Sinderen, D. (1999). Molecular Characterization of a Phage-Encoded Resistance System in Lactococcus lactis. Appl. Environ. Microbiol. 65: 1891-1899 [Abstract] [Full Text]  
  • Emond, E., Dion, E., Walker, S. A., Vedamuthu, E. R., Kondo, J. K., Moineau, S. (1998). AbiQ, an Abortive Infection Mechanism from Lactococcus lactis. Appl. Environ. Microbiol. 64: 4748-4756 [Abstract] [Full Text]  
  • Walker, S. A., Dombroski, C. S., Klaenhammer, T. R. (1998). Common Elements Regulating Gene Expression in Temperate and Lytic Bacteriophages of Lactococcus Species. Appl. Environ. Microbiol. 64: 1147-1152 [Abstract] [Full Text]  
  • Walker, S. A., Klaenhammer, T. R. (1998). Molecular Characterization of a Phage-Inducible Middle Promoter and Its Transcriptional Activator from the Lactococcal Bacteriophage phi 31. J. Bacteriol. 180: 921-931 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»