This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by MacGregor, B. J.
Right arrow Articles by Donohue, T. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by MacGregor, B. J.
Right arrow Articles by Donohue, T. J.

Next Article 

J. Bacteriol., Jan 1998, 1-9, Vol 180, No. 1
Copyright © 1998, American Society for Microbiology

Transcription of the Rhodobacter sphaeroides cycA P1 promoter by alternate RNA polymerase holoenzymes [In Process Citation]

BJ MacGregor, RK Karls and TJ Donohue
Department of Bacteriology, University of Wisconsin-Madison, 53706, USA.

These experiments sought to identify what form of RNA polymerase transcribes the P1 promoter for the Rhodobacter sphaeroides cytochrome c2 gene (cycA). In vitro, cycA P1 was recognized by an RNA polymerase holoenzyme fraction that transcribes several well-characterized Escherichia coli heat shock (sigma32) promoters. The in vivo effects of mutations flanking the transcription initiation site (+1) also suggested that cycA P1 was recognized by an RNA polymerase similar to E. coli Esigma32. Function of cycA P1 was not altered by mutations more than 35 bp upstream of position +1 or by alterations downstream of -7. A point mutation at position -34 that is towards the E. coli Esigma32 - 35 consensus sequence (G34T) increased cycA P1 activity approximately 20-fold, while several mutations that reduced or abolished promoter function changed highly conserved bases in presumed -10 or -35 elements. In addition, cycA P1 function was retained in mutant promoters with a spacer region as short as 14 nucleotides. When either wild-type or G34T promoters were incubated with reconstituted RNA polymerase holoenzymes, cycA P1 transcription was observed only with samples containing either a 37-kDa subunit that is a member of the heat shock sigma factor family (Esigma37) or a 38-kDa subunit that also allows core RNA polymerase to recognize E. coli heat shock promoters (Esigma38). (R. K. Karls, J. Brooks, P. Rossmeissl, J. Luedke, and T. J. Donohue, J. Bacteriol. 180:10-19, 1998).


This article has been cited by other articles:

  • Green, H. A., Donohue, T. J. (2006). Activity of Rhodobacter sphaeroides RpoHII, a Second Member of the Heat Shock Sigma Factor Family.. J. Bacteriol. 188: 5712-5721 [Abstract] [Full Text]  
  • Anthony, J. R., Warczak, K. L., Donohue, T. J. (2005). A transcriptional response to singlet oxygen, a toxic byproduct of photosynthesis. Proc. Natl. Acad. Sci. USA 102: 6502-6507 [Abstract] [Full Text]  
  • Braatsch, S., Moskvin, O. V., Klug, G., Gomelsky, M. (2004). Responses of the Rhodobacter sphaeroides Transcriptome to Blue Light under Semiaerobic Conditions. J. Bacteriol. 186: 7726-7735 [Abstract] [Full Text]  
  • Comolli, J. C., Carl, A. J., Hall, C., Donohue, T. (2002). Transcriptional Activation of the Rhodobacter sphaeroides Cytochrome c2 Gene P2 Promoter by the Response Regulator PrrA. J. Bacteriol. 184: 390-399 [Abstract] [Full Text]  
  • Karls, R. K., Brooks, J., Rossmeissl, P., Luedke, J., Donohue, T. J. (1998). Metabolic Roles of a Rhodobacter sphaeroides Member of the sigma 32 Family. J. Bacteriol. 180: 10-19 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»