This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Allen, A. G.
Right arrow Articles by Maskell, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Allen, A. G.
Right arrow Articles by Maskell, D. J.

 Previous Article  |  Next Article 

J. Bacteriol., Jan 1998, 35-40, Vol 180, No. 1
Copyright © 1998, American Society for Microbiology

Identification and cloning of waaF (rfaF) from Bordetella pertussis and use to generate mutants of Bordetella spp. with deep rough lipopolysaccharide [In Process Citation]

AG Allen, T Isobe and DJ Maskell
Centre for Veterinary Science, Department of Clinical Veterinary Medicine, University of Cambridge, United Kingdom. aga20@cam.ac.uk

A DNA locus from Bordetella pertussis capable of reconstituting lipopolysaccharide (LPS) O-antigen biosynthesis in Salmonella typhimurium SL3789 (rfaF511) has been isolated, by using selection with the antibiotic novobiocin. DNA within the locus encodes a protein with amino acid sequence similarity to heptosyltransferase II, encoded by waaF (previously rfaF) in other gram-negative bacteria. Mutation of this gene in B. pertussis, Bordetella parapertussis, and Bordetella bronchiseptica by allelic exchange generated bacteria with deep rough LPS phenotypes consistent with the proposed function of the gene as an inner core heptosyltransferase. These are the first LPS mutants generated in B. parapertussis and B. bronchiseptica and the first deep rough mutants of any of the bordetellae.


This article has been cited by other articles:

  • Geurtsen, J., Dzieciatkowska, M., Steeghs, L., Hamstra, H.-J., Boleij, J., Broen, K., Akkerman, G., el Hassan, H., Li, J., Richards, J. C., Tommassen, J., van der Ley, P. (2009). Identification of a Novel Lipopolysaccharide Core Biosynthesis Gene Cluster in Bordetella pertussis, and Influence of Core Structure and Lipid A Glucosamine Substitution on Endotoxic Activity. Infect. Immun. 77: 2602-2611 [Abstract] [Full Text]  
  • Gronow, S., Brabetz, W., Lindner, B., Brade, H. (2005). OpsX from Haemophilus influenzae Represents a Novel Type of Heptosyltransferase I in Lipopolysaccharide Biosynthesis. J. Bacteriol. 187: 6242-6247 [Abstract] [Full Text]  
  • Schaeffer, L. M., McCormack, F. X., Wu, H., Weiss, A. A. (2004). Interactions of Pulmonary Collectins with Bordetella bronchiseptica and Bordetella pertussis Lipopolysaccharide Elucidate the Structural Basis of Their Antimicrobial Activities. Infect. Immun. 72: 7124-7130 [Abstract] [Full Text]  
  • Schaeffer, L. M., McCormack, F. X., Wu, H., Weiss, A. A. (2004). Bordetella pertussis Lipopolysaccharide Resists the Bactericidal Effects of Pulmonary Surfactant Protein A. J. Immunol. 173: 1959-1965 [Abstract] [Full Text]  
  • Fuchslocher, B., Millar, L. L., Cotter, P. A. (2003). Comparison of bipA Alleles within and across Bordetella Species. Infect. Immun. 71: 3043-3052 [Abstract] [Full Text]  
  • Moxon, R., Rappuoli, R. (2002). Bacterial pathogen genomics and vaccines. Br Med Bull 62: 45-58 [Abstract] [Full Text]  
  • Oldfield, N. J., Moran, A. P., Millar, L. A., Prendergast, M. M., Ketley, J. M. (2002). Characterization of the Campylobacter jejuni Heptosyltransferase II Gene, waaF, Provides Genetic Evidence that Extracellular Polysaccharide Is Lipid A Core Independent. J. Bacteriol. 184: 2100-2107 [Abstract] [Full Text]  
  • Preston, A., Maskell, D. (2001). Invited review: The molecular genetics and role in infection of lipopolysaccharide biosynthesis in the Bordetellae. Innate Immunity 7: 251-261  
  • Gronow, S., Brade, H. (2001). Invited review: Lipopolysaccharide biosynthesis: which steps do bacteria need to survive?. Innate Immunity 7: 3-23 [Abstract]  
  • Harvill, E. T., Preston, A., Cotter, P. A., Allen, A. G., Maskell, D. J., Miller, J. F. (2000). Multiple Roles for Bordetella Lipopolysaccharide Molecules during Respiratory Tract Infection. Infect. Immun. 68: 6720-6728 [Abstract] [Full Text]  
  • West, N. P., Jungnitz, H., Fitter, J. T., McArthur, J. D., Guzman, C. A., Walker, M. J. (2000). Role of Phosphoglucomutase of Bordetella bronchiseptica in Lipopolysaccharide Biosynthesis and Virulence. Infect. Immun. 68: 4673-4680 [Abstract] [Full Text]  
  • Bauer, B. A., Lumbley, S. R., Hansen, E. J. (1999). Characterization of a WaaF (RfaF) Homolog Expressed by Haemophilus ducreyi. Infect. Immun. 67: 899-907 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»