This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chung, Y. S.
Right arrow Articles by Dubnau, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chung, Y. S.
Right arrow Articles by Dubnau, D.

 Previous Article  |  Next Article 

J. Bacteriol., 01 1998, 41-45, Vol 180, No. 1
Copyright © 1998, American Society for Microbiology

All seven comG open reading frames are required for DNA binding during transformation of competent Bacillus subtilis [In Process Citation]

YS Chung and D Dubnau
Public Health Research Institute, New York, New York 10016, USA.

The seven proteins encoded by the comG operon of Bacillus subtilis exhibit similarity to gene products required for the assembly of type 4 pili and for the secretion of certain proteins in gram-negative bacteria. Although polar transposon insertions in comG result in the loss of transformability and in the failure of cells grown through the competence regimen to bind DNA, it was not known whether the ComG proteins are all required for competence. We have constructed strains missing each of these proteins individually and found that they are all nontransformable and fail to bind transforming DNA to the cell surface. The implications of these findings are discussed.


This article has been cited by other articles:

  • Gardan, R., Besset, C., Guillot, A., Gitton, C., Monnet, V. (2009). The Oligopeptide Transport System Is Essential for the Development of Natural Competence in Streptococcus thermophilus Strain LMD-9. J. Bacteriol. 191: 4647-4655 [Abstract] [Full Text]  
  • Niu, G., Okinaga, T., Zhu, L., Banas, J., Qi, F., Merritt, J. (2008). Characterization of irvR, a Novel Regulator of the irvA-Dependent Pathway Required for Genetic Competence and Dextran-Dependent Aggregation in Streptococcus mutans. J. Bacteriol. 190: 7268-7274 [Abstract] [Full Text]  
  • Jeon, B., Zhang, Q. (2007). Cj0011c, a Periplasmic Single- and Double-Stranded DNA-Binding Protein, Contributes to Natural Transformation in Campylobacter jejuni. J. Bacteriol. 189: 7399-7407 [Abstract] [Full Text]  
  • Sibbald, M. J. J. B., Ziebandt, A. K., Engelmann, S., Hecker, M., de Jong, A., Harmsen, H. J. M., Raangs, G. C., Stokroos, I., Arends, J. P., Dubois, J. Y. F., van Dijl, J. M. (2006). Mapping the Pathways to Staphylococcal Pathogenesis by Comparative Secretomics. Microbiol. Mol. Biol. Rev. 70: 755-788 [Abstract] [Full Text]  
  • Chen, I., Provvedi, R., Dubnau, D. (2006). A Macromolecular Complex Formed by a Pilin-like Protein in Competent Bacillus subtilis. J. Biol. Chem. 281: 21720-21727 [Abstract] [Full Text]  
  • Lalucat, J., Bennasar, A., Bosch, R., Garcia-Valdes, E., Palleroni, N. J. (2006). Biology of Pseudomonas stutzeri. Microbiol. Mol. Biol. Rev. 70: 510-547 [Abstract] [Full Text]  
  • Chen, I., Christie, P. J., Dubnau, D. (2005). The Ins and Outs of DNA Transfer in Bacteria. Science 310: 1456-1460 [Abstract] [Full Text]  
  • Petersen, F. C., Tao, L., Scheie, A. A. (2005). DNA Binding-Uptake System: a Link between Cell-to-Cell Communication and Biofilm Formation. J. Bacteriol. 187: 4392-4400 [Abstract] [Full Text]  
  • Merritt, J., Qi, F., Shi, W. (2005). A unique nine-gene comY operon in Streptococcus mutans. Microbiology 151: 157-166 [Abstract] [Full Text]  
  • Wiesner, R. S., Hendrixson, D. R., DiRita, V. J. (2003). Natural Transformation of Campylobacter jejuni Requires Components of a Type II Secretion System. J. Bacteriol. 185: 5408-5418 [Abstract] [Full Text]  
  • Okamoto, S., Ohmori, M. (2002). The Cyanobacterial PilT Protein Responsible for Cell Motility and Transformation Hydrolyzes ATP. Plant Cell Physiol 43: 1127-1136 [Abstract] [Full Text]  
  • Mader, U., Homuth, G., Scharf, C., Buttner, K., Bode, R., Hecker, M. (2002). Transcriptome and Proteome Analysis of Bacillus subtilis Gene Expression Modulated by Amino Acid Availability. J. Bacteriol. 184: 4288-4295 [Abstract] [Full Text]  
  • Ogura, M., Yamaguchi, H., Kobayashi, K., Ogasawara, N., Fujita, Y., Tanaka, T. (2002). Whole-Genome Analysis of Genes Regulated by the Bacillus subtilis Competence Transcription Factor ComK. J. Bacteriol. 184: 2344-2351 [Abstract] [Full Text]  
  • Erlendsson, L. S., Hederstedt, L. (2002). Mutations in the Thiol-Disulfide Oxidoreductases BdbC and BdbD Can Suppress Cytochrome c Deficiency of CcdA-Defective Bacillus subtilis Cells. J. Bacteriol. 184: 1423-1429 [Abstract] [Full Text]  
  • Blank, T. E., Donnenberg, M. S. (2001). Novel Topology of BfpE, a Cytoplasmic Membrane Protein Required for Type IV Fimbrial Biogenesis in Enteropathogenic Escherichia coli. J. Bacteriol. 183: 4435-4450 [Abstract] [Full Text]  
  • Breuner, A., Hammer, K. (2001). Resolvase-like recombination performed by the TP901-1 integrase. Microbiology 147: 2051-2063 [Abstract] [Full Text]  
  • Herd, M., Kocks, C. (2001). Gene Fragments Distinguishing an Epidemic-Associated Strain from a Virulent Prototype Strain of Listeria monocytogenes Belong to a Distinct Functional Subset of Genes and Partially Cross-Hybridize with Other Listeria Species. Infect. Immun. 69: 3972-3979 [Abstract] [Full Text]  
  • Chen, I., Gotschlich, E. C. (2001). ComE, a Competence Protein from Neisseria gonorrhoeae with DNA-Binding Activity. J. Bacteriol. 183: 3160-3168 [Abstract] [Full Text]  
  • Graupner, S., Wackernagel, W. (2001). Pseudomonas stutzeri Has Two Closely Related pilA Genes (Type IV Pilus Structural Protein) with Opposite Influences on Natural Genetic Transformation. J. Bacteriol. 183: 2359-2366 [Abstract] [Full Text]  
  • Planet, P. J., Kachlany, S. C., DeSalle, R., Figurski, D. H. (2001). Phylogeny of genes for secretion NTPases: Identification of the widespread tadA subfamily and development of a diagnostic key for gene classification. Proc. Natl. Acad. Sci. USA 98: 2503-2508 [Abstract] [Full Text]  
  • Tjalsma, H., Bolhuis, A., Jongbloed, J. D. H., Bron, S., van Dijl, J. M. (2000). Signal Peptide-Dependent Protein Transport in Bacillus subtilis: a Genome-Based Survey of the Secretome. Microbiol. Mol. Biol. Rev. 64: 515-547 [Abstract] [Full Text]  
  • Ogura, M., Tanaka, T. (2000). Bacillus subtilis comZ (yjzA) Negatively Affects Expression of comG but Not comK. J. Bacteriol. 182: 4992-4994 [Abstract] [Full Text]  
  • Bolhuis, A., Venema, G., Quax, W. J., Bron, S., van Dijl, J. M. (1999). Functional Analysis of Paralogous Thiol-disulfide Oxidoreductases in Bacillus subtilis. J. Biol. Chem. 274: 24531-24538 [Abstract] [Full Text]  
  • Liu, J., Zuber, P. (1998). A Molecular Switch Controlling Competence and Motility: Competence Regulatory Factors ComS, MecA, and ComK Control sigma D-Dependent Gene Expression in Bacillus subtilis. J. Bacteriol. 180: 4243-4251 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»