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J Bacteriol, May 1998, p. 2749-2755, Vol. 180, No. 10
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Unconventional Genomic Organization in the Alpha Subgroup of the Proteobacteria

Estelle Jumas-Bilak, Sylvie Michaux-Charachon, Gisele Bourg, Michel Ramuz, and Annick Allardet-Servent*

Faculté de Médecine, Institut National de la Santé et de la Recherche Médicale, Unité 431, 30900 Nimes, France

Received 14 November 1997/Accepted 2 March 1998

Pulsed-field gel electrophoresis was used to analyze the genomic organization of 16 bacteria belonging or related to the family Rhizobiaceae of the alpha subgroup of the class Proteobacteria. The number and sizes of replicons were determined by separating nondigested DNA. Hybridization of an rrn gene probe was used to distinguish between chromosomes and plasmids. Members of the genus Agrobacterium all possess two chromosomes, and each biovar has a specific genome size. As previously demonstrated for Agrobacterium tumefaciens C58, the smaller chromosomes of Agrobacterium biovar 1 and Agrobacterium rubi strains appear to be linear. The genomes of Rhizobium strains were all of similar sizes but were seen to contain either one, two, or three megareplicons. Only one chromosome was present in the member of the related genus Phyllobacterium. We found one or two chromosomes in Rhodobacter and Brucella species, two chromosomes in Ochrobactrum anthropi, and one chromosome in Mycoplana dimorpha and Bartonella quintana; all of these genera are related to the Rhizobiaceae. The presence of multiple chromosomes is discussed from a phylogenetic and taxonomic point of view.

* Corresponding author. Mailing address: Faculté de Médecine, Institut National de la Santé et de la Recherche Médicale, Unité 431, Ave. Kennedy, 30900 Nimes, France. Phone: (33) 4 66 23 46 79. Fax: (33) 4 66 23 66 52. E-mail: docallaghan{at}zeus.sc.univ-montp1.fr.

J Bacteriol, May 1998, p. 2749-2755, Vol. 180, No. 10
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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