This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mouncey, N. J.
Right arrow Articles by Kaplan, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mouncey, N. J.
Right arrow Articles by Kaplan, S.

 Previous Article  |  Next Article 

J Bacteriol, June 1998, p. 2924-2930, Vol. 180, No. 11
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Cascade Regulation of Dimethyl Sulfoxide Reductase (dor) Gene Expression in the Facultative Phototroph Rhodobacter sphaeroides 2.4.1T

Nigel J. Mouncey and Samuel Kaplan*

Department of Microbiology and Molecular Genetics, The University of Texas Health Science Center Medical School, Houston, Texas 77030

Received 30 December 1997/Accepted 25 March 1998

Under anaerobic-dark growth conditions, in the presence of the alternative electron acceptor dimethyl sulfoxide (DMSO) or trimethylamine N-oxide (TMAO), Rhodobacter sphaeroides 2.4.1T respires anaerobically using the molybdoenzyme DMSO reductase (DMSOR). Genes encoding DMSOR and associated proteins are encoded by genes of the dor locus. Previously, we demonstrated that the expression of DMSOR is regulated by both the oxygen status of the cell via the FnrL protein and by the presence of DMSO or TMAO, presumably through the DorS-DorR two-component sensor-regulator system. Here we further investigate expression of the dor genes through the use of transcriptional lacZ fusions to the dorS, dorR, and dorC promoters. The expression of dorC::lacZ was strongly induced by the absence of oxygen and presence of DMSO. In accordance with our previous findings of DMSOR activity, dorC::lacZ expression was reduced by up to one-third when cells were grown photosynthetically in the presence of DMSO with medium or high light, compared to the expression observed after anaerobic-dark growth. The induction of dorC::lacZ expression in the presence of DMSO was dependent on the DorS and DorR proteins. Expression of the dorS and dorR genes was also induced in the absence of oxygen. In an FnrL mutant, dorS::lacZ expression was not induced when oxygen tensions in the media were lowered, in contrast to what occurred in the wild-type strain. The expression of dorS::lacZ and dorR::lacZ was dependent on the DorS and DorR proteins themselves, suggesting the importance of autoregulation. These results demonstrate a cascade regulation of dor gene expression, where the expression of the regulatory proteins DorS and DorR governs the downstream regulation of the dorCBA operon encoding the structural proteins of DMSOR.

* Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics, The University of Texas Health Science Center Medical School, 6431 Fannin, Houston, TX 77030. Phone: (713) 500-5502. Fax: (713) 500-5499. E-mail: skaplan{at}utmmg.med.uth.tmc.edu.

J Bacteriol, June 1998, p. 2924-2930, Vol. 180, No. 11
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Gomelsky, L., Moskvin, O. V., Stenzel, R. A., Jones, D. F., Donohue, T. J., Gomelsky, M. (2008). Hierarchical Regulation of Photosynthesis Gene Expression by the Oxygen-Responsive PrrBA and AppA-PpsR Systems of Rhodobacter sphaeroides. J. Bacteriol. 190: 8106-8114 [Abstract] [Full Text]  
  • Zeilstra-Ryalls, J. H., Schornberg, K. L. (2006). Analysis of hemF Gene Function and Expression in Rhodobacter sphaeroides 2.4.1. J. Bacteriol. 188: 801-804 [Abstract] [Full Text]  
  • Tavano, C. L., Podevels, A. M., Donohue, T. J. (2005). Identification of Genes Required for Recycling Reducing Power during Photosynthetic Growth. J. Bacteriol. 187: 5249-5258 [Abstract] [Full Text]  
  • Pappas, C. T., Sram, J., Moskvin, O. V., Ivanov, P. S., Mackenzie, R. C., Choudhary, M., Land, M. L., Larimer, F. W., Kaplan, S., Gomelsky, M. (2004). Construction and Validation of the Rhodobacter sphaeroides 2.4.1 DNA Microarray: Transcriptome Flexibility at Diverse Growth Modes. J. Bacteriol. 186: 4748-4758 [Abstract] [Full Text]  
  • Tavano, C. L., Comolli, J. C., Donohue, T. J. (2004). The role of dor gene products in controlling the P2 promoter of the cytochrome c2 gene, cycA, in Rhodobacter sphaeroides. Microbiology 150: 1893-1899 [Abstract] [Full Text]  
  • Kappler, U., Huston, W. M., McEwan, A. G. (2002). Control of dimethylsulfoxide reductase expression in Rhodobacter capsulatus: the role of carbon metabolites and the response regulators DorR and RegA. Microbiology 148: 605-614 [Abstract] [Full Text]  
  • Yamamoto, I., Ujiiye, T., Ohshima, Y., Satoh, T. (2001). Mutational Analysis of Regulatory cis-acting Elements for the Transcriptional Activation of the dmsCBA Operon in Rhodobacter sphaeroides f. sp. denitrificans. Plant Cell Physiol 42: 703-709 [Abstract] [Full Text]  
  • Oh, J.-I., Eraso, J. M., Kaplan, S. (2000). Interacting Regulatory Circuits Involved in Orderly Control of Photosynthesis Gene Expression in Rhodobacter sphaeroides 2.4.1. J. Bacteriol. 182: 3081-3087 [Abstract] [Full Text]  
  • Ansaldi, M., Simon, G., Lepelletier, M., Méjean, V. (2000). The TorR High-Affinity Binding Site Plays a Key Role in Both torR Autoregulation and torCAD Operon Expression in Escherichia coli. J. Bacteriol. 182: 961-966 [Abstract] [Full Text]  
  • Mouncey, N. J., Kaplan, S. (1998). Redox-Dependent Gene Regulation in Rhodobacter sphaeroides 2.4.1T: Effects on Dimethyl Sulfoxide Reductase (dor) Gene Expression. J. Bacteriol. 180: 5612-5618 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»