This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schwartz, E.
Right arrow Articles by Friedrich, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schwartz, E.
Right arrow Articles by Friedrich, B.

 Previous Article  |  Next Article 

J Bacteriol, June 1998, p. 3197-3204, Vol. 180, No. 12
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Transcriptional Regulation of Alcaligenes eutrophus Hydrogenase Genes

Edward Schwartz,* Ulrike Gerischer,dagger and Bärbel Friedrich

Institut für Biologie der Humboldt-Universität zu Berlin and Institut für Pflanzenphysiologie und Mikrobiologie der Freien Universität Berlin, Berlin, Germany

Received 21 January 1998/Accepted 8 April 1998

Alcaligenes eutrophus H16 produces a soluble hydrogenase (SH) and a membrane-bound hydrogenase (MBH) which catalyze the oxidation of H2, supplying the organism with energy for autotrophic growth. The promoters of the structural genes for the SH and the MBH, PSH and PMBH, respectively, were identified by means of the primer extension technique. Both promoters were active in vivo under hydrogenase-derepressing conditions but directed only low levels of transcription under conditions which repressed hydrogenase synthesis. The cellular pools of SH and MBH transcripts under the different growth conditions correlated with the activities of the respective promoters. Also, an immediate and drastic increase in transcript pool levels occurred upon derepression of the hydrogenase system. Both promoters were dependent on the minor sigma factor sigma 54 and on the hydrogenase regulator HoxA in vivo. PSH was stronger than PMBH under both heterotrophic and autotrophic growth conditions. The two promoters were induced at approximately the same rates upon derepression of the hydrogenase system in diauxic cultures. The response regulator HoxA mediated low-level activation of PSH and PMBH in a heterologous system.

* Corresponding author. Mailing address: Institut für Biologie, Mikrobiologie, Humboldt-Universität zu Berlin, Chausseestr. 117, D-10115 Berlin, Germany. Phone: 49-30-2093-8117. Fax: 49-30-2093-8102. E-mail: edward=schwartz{at}biologie.hu-berlin.de.

dagger Present address: Abteilung Angewandte Mikrobiologie, Universität Ulm, D-89069 Ulm, Germany.

J Bacteriol, June 1998, p. 3197-3204, Vol. 180, No. 12
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Saggu, M., Zebger, I., Ludwig, M., Lenz, O., Friedrich, B., Hildebrandt, P., Lendzian, F. (2009). Spectroscopic Insights into the Oxygen-tolerant Membrane-associated [NiFe] Hydrogenase of Ralstonia eutropha H16. J. Biol. Chem. 284: 16264-16276 [Abstract] [Full Text]  
  • Ludwig, M., Schubert, T., Zebger, I., Wisitruangsakul, N., Saggu, M., Strack, A., Lenz, O., Hildebrandt, P., Friedrich, B. (2009). Concerted Action of Two Novel Auxiliary Proteins in Assembly of the Active Site in a Membrane-bound [NiFe] Hydrogenase. J. Biol. Chem. 284: 2159-2168 [Abstract] [Full Text]  
  • Wen, Y., Feng, J., Scott, D. R., Marcus, E. A, Sachs, G. (2009). The pH-Responsive Regulon of HP0244 (FlgS), the Cytoplasmic Histidine Kinase of Helicobacter pylori. J. Bacteriol. 191: 449-460 [Abstract] [Full Text]  
  • Ludwig, M., Cracknell, J. A., Vincent, K. A., Armstrong, F. A., Lenz, O. (2009). Oxygen-tolerant H2 Oxidation by Membrane-bound [NiFe] Hydrogenases of Ralstonia Species: COPING WITH LOW LEVEL H2 IN AIR. J. Biol. Chem. 284: 465-477 [Abstract] [Full Text]  
  • Norton, J. M., Klotz, M. G., Stein, L. Y., Arp, D. J., Bottomley, P. J., Chain, P. S. G., Hauser, L. J., Land, M. L., Larimer, F. W., Shin, M. W., Starkenburg, S. R. (2008). Complete Genome Sequence of Nitrosospira multiformis, an Ammonia-Oxidizing Bacterium from the Soil Environment. Appl. Environ. Microbiol. 74: 3559-3572 [Abstract] [Full Text]  
  • Strube, K., de Vries, S., Cramm, R. (2007). Formation of a Dinitrosyl Iron Complex by NorA, a Nitric Oxide-binding Di-iron Protein from Ralstonia eutropha H16. J. Biol. Chem. 282: 20292-20300 [Abstract] [Full Text]  
  • Mascher, T., Helmann, J. D., Unden, G. (2006). Stimulus Perception in Bacterial Signal-Transducing Histidine Kinases. Microbiol. Mol. Biol. Rev. 70: 910-938 [Abstract] [Full Text]  
  • Rey, F. E., Oda, Y., Harwood, C. S. (2006). Regulation of Uptake Hydrogenase and Effects of Hydrogen Utilization on Gene Expression in Rhodopseudomonas palustris.. J. Bacteriol. 188: 6143-6152 [Abstract] [Full Text]  
  • Lenz, O., Gleiche, A., Strack, A., Friedrich, B. (2005). Requirements for Heterologous Production of a Complex Metalloenzyme: the Membrane-Bound [NiFe] Hydrogenase. J. Bacteriol. 187: 6590-6595 [Abstract] [Full Text]  
  • Buhrke, T., Lenz, O., Krauss, N., Friedrich, B. (2005). Oxygen Tolerance of the H2-sensing [NiFe] Hydrogenase from Ralstonia eutropha H16 Is Based on Limited Access of Oxygen to the Active Site. J. Biol. Chem. 280: 23791-23796 [Abstract] [Full Text]  
  • Burgdorf, T., van der Linden, E., Bernhard, M., Yin, Q. Y., Back, J. W., Hartog, A. F., Muijsers, A. O., de Koster, C. G., Albracht, S. P. J., Friedrich, B. (2005). The Soluble NAD+-Reducing [NiFe]-Hydrogenase from Ralstonia eutropha H16 Consists of Six Subunits and Can Be Specifically Activated by NADPH. J. Bacteriol. 187: 3122-3132 [Abstract] [Full Text]  
  • Busch, A., Pohlmann, A., Friedrich, B., Cramm, R. (2004). A DNA Region Recognized by the Nitric Oxide-Responsive Transcriptional Activator NorR Is Conserved in {beta}- and {gamma}-Proteobacteria. J. Bacteriol. 186: 7980-7987 [Abstract] [Full Text]  
  • Bleijlevens, B., Buhrke, T., van der Linden, E., Friedrich, B., Albracht, S. P. J. (2004). The Auxiliary Protein HypX Provides Oxygen Tolerance to the Soluble [NiFe]-Hydrogenase of Ralstonia eutropha H16 by Way of a Cyanide Ligand to Nickel. J. Biol. Chem. 279: 46686-46691 [Abstract] [Full Text]  
  • Gliese, N., Khodaverdi, V., Schobert, M., Gorisch, H. (2004). AgmR controls transcription of a regulon with several operons essential for ethanol oxidation in Pseudomonas aeruginosa ATCC 17933. Microbiology 150: 1851-1857 [Abstract] [Full Text]  
  • Elsen, S., Duche, O., Colbeau, A. (2003). Interaction between the H2 Sensor HupUV and the Histidine Kinase HupT Controls HupSL Hydrogenase Synthesis in Rhodobacter capsulatus. J. Bacteriol. 185: 7111-7119 [Abstract] [Full Text]  
  • Buhrke, T., Bleijlevens, B., Albracht, S. P. J., Friedrich, B. (2001). Involvement of hyp Gene Products in Maturation of the H2-Sensing [NiFe] Hydrogenase of Ralstonia eutropha. J. Bacteriol. 183: 7087-7093 [Abstract] [Full Text]  
  • Trautwein, G., Gerischer, U. (2001). Effects Exerted by Transcriptional Regulator PcaU from Acinetobacter sp. Strain ADP1. J. Bacteriol. 183: 873-881 [Abstract] [Full Text]  
  • Schobert, M., Görisch, H. (2001). A soluble two-component regulatory system controls expression of quinoprotein ethanol dehydrogenase (QEDH) but not expression of cytochrome c550 of the ethanol-oxidation system in Pseudomonas aeruginosa. Microbiology 147: 363-372 [Abstract] [Full Text]  
  • Saito, A., Ishizaka, M., Francisco, P. B. Jr, Fujii, T., Miyashita, K. (2000). Transcriptional co-regulation of five chitinase genes scattered on the Streptomyces coelicolor A3(2) chromosome. Microbiology 146: 2937-2946 [Abstract] [Full Text]  
  • Kleihues, L., Lenz, O., Bernhard, M., Buhrke, T., Friedrich, B. (2000). The H2 Sensor of Ralstonia eutropha Is a Member of the Subclass of Regulatory [NiFe] Hydrogenases. J. Bacteriol. 182: 2716-2724 [Abstract] [Full Text]  
  • Happe, T., Schütz, K., Böhme, H. (2000). Transcriptional and Mutational Analysis of the Uptake Hydrogenase of the Filamentous Cyanobacterium Anabaena variabilis ATCC 29413. J. Bacteriol. 182: 1624-1631 [Abstract] [Full Text]  
  • Schwartz, E., Buhrke, T., Gerischer, U., Friedrich, B. (1999). Positive Transcriptional Feedback Controls Hydrogenase Expression in Alcaligenes eutrophus H16. J. Bacteriol. 181: 5684-5692 [Abstract] [Full Text]  
  • Siedow, A., Cramm, R., Siddiqui, R. A., Friedrich, B. (1999). A Megaplasmid-Borne Anaerobic Ribonucleotide Reductase in Alcaligenes eutrophus H16. J. Bacteriol. 181: 4919-4928 [Abstract] [Full Text]  
  • Lenz, O., Friedrich, B. (1998). A novel multicomponent regulatory system mediates H2 sensing in Alcaligenes eutrophus. Proc. Natl. Acad. Sci. USA 95: 12474-12479 [Abstract] [Full Text]  
  • Bernhard, M., Buhrke, T., Bleijlevens, B., De Lacey, A. L., Fernandez, V. M., Albracht, S. P. J., Friedrich, B. (2001). The H2 Sensor of Ralstonia eutropha. BIOCHEMICAL CHARACTERISTICS, SPECTROSCOPIC PROPERTIES, AND ITS INTERACTION WITH A HISTIDINE PROTEIN KINASE. J. Biol. Chem. 276: 15592-15597 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»