Previous Article | Next Article 
Vol. 180, Issue 13, 3330-3338, July 1, 1998
1 Department of Entomology and Plant Pathology,
Oklahoma State University, Stillwater, Oklahoma
74078-3032,1 and
2 Plant Improvement
Division, Horticulture and Food Research Institute of New Zealand,
Auckland, New Zealand2
Coronafacic acid (CFA) is the polyketide component of coronatine
(COR), a phytotoxin produced by the plant-pathogenic bacterium Pseudomonas syringae. The genes involved in CFA
biosynthesis are encoded by a single transcript which encompasses 19 kb
of the COR gene cluster. In the present study, the nucleotide sequence was determined for a 4-kb region located at the 3' end of the CFA
biosynthetic gene cluster. Three open reading frames were identified
and designated cfa8, cfa9, and
tnp1; the predicted translation products of these genes
showed relatedness to oxidoreductases, thioesterases, and transposases,
respectively. The translational products of cfa8 and
cfa9 were overproduced in Escherichia coli BL21; however, tnp1 was not translated in these
experiments. Mutagenesis and complementation analysis indicated that
cfa8 is required for the production of CFA and COR.
Analysis of a cfa9 mutant indicated that this gene is
dispensable for CFA and COR production but may increase the release of
enzyme-bound products from the COR pathway; tnp1, however,
had no obvious function in CFA or COR biosynthesis. A genetic strategy
was used to produce CFA in a P. syringae strain which lacks
the COR gene cluster; this approach will be useful in future studies
designed to investigate biosynthetic products of the CFA gene cluster.
Analysis of Genes Involved in Biosynthesis of
Coronafacic Acid, the Polyketide Component of the Phytotoxin
Coronatine
Copyright © 1998 by American Society for Microbiology
This article has been cited by other articles:
-
Li, C., Florova, G., Akopiants, K., Reynolds, K. A.
(2004). Crotonyl-coenzyme A reductase provides methylmalonyl-CoA precursors for monensin biosynthesis by Streptomyces cinnamonensis in an oil-based extended fermentation. Microbiology
150: 3463-3472
[Abstract] [Full Text] -
Sesma, A., Sundin, G. W., Murillo, J.
(2000). Phylogeny of the replication regions of pPT23A-like plasmids from Pseudomonas syringae. Microbiology
146: 2375-2384
[Abstract] [Full Text] -
Peñaloza-Vázquez, A., Preston, G. M., Collmer, A., Bender, C. L.
(2000). Regulatory interactions between the Hrp type III protein secretion system and coronatine biosynthesis in Pseudomonas syringae pv. tomato DC3000. Microbiology
146: 2447-2456
[Abstract] [Full Text] -
Turner, M. S., Helmann, J. D.
(2000). Mutations in Multidrug Efflux Homologs, Sugar Isomerases, and Antimicrobial Biosynthesis Genes Differentially Elevate Activity of the sigma X and sigma W Factors in Bacillus subtilis. J. Bacteriol.
182: 5202-5210
[Abstract] [Full Text] -
Liu, H., Reynolds, K. A.
(1999). Role of Crotonyl Coenzyme A Reductase in Determining the Ratio of Polyketides Monensin A and Monensin B Produced by Streptomyces cinnamonensis. J. Bacteriol.
181: 6806-6813
[Abstract] [Full Text] -
Bender, C. L., Alarcon-Chaidez, F., Gross, D. C.
(1999). Pseudomonas syringae Phytotoxins: Mode of Action, Regulation, and Biosynthesis by Peptide and Polyketide Synthetases. Microbiol. Mol. Biol. Rev.
63: 266-292
[Abstract] [Full Text] -
Bangera, M. G., Thomashow, L. S.
(1999). Identification and Characterization of a Gene Cluster for Synthesis of the Polyketide Antibiotic 2,4-Diacetylphloroglucinol from Pseudomonas fluorescens Q2-87. J. Bacteriol.
181: 3155-3163
[Abstract] [Full Text] -
Rangaswamy, V., Jiralerspong, S., Parry, R., Bender, C. L.
(1998). Biosynthesis of the Pseudomonas polyketide coronafacic acid requires monofunctional and multifunctional polyketide synthase proteins. Proc. Natl. Acad. Sci. USA
95: 15469-15474
[Abstract] [Full Text]
Copyright © 2009 by the American Society for Microbiology. For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»