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Vol. 180, Issue 13, 3480-3482, July 1, 1998

NOTE
Mutants of Citrobacter freundii That Transport and Utilize Melibiose

Noriko Okazaki1, Xing Jue Xu1, Toshi Shimamoto1, Masayuki Kuroda2, Thomas H. Wilson3, and Tomofusa Tsuchiya12

1 Department of Microbiology, Faculty of Pharmaceutical Sciences,1 and 2 Gene Research Center,2 Okayama University, Tsushima, Okayama 700, Japan, and 3 Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 021153

We have isolated mutants of Citrobacter freundii that can grow on melibiose. Inducible alpha -galactosidase activity and melibiose transport activity were detected in the mutant cells but not in the wild-type cells. We detected a DNA region which hybridized with melB (the gene for the melibiose transporter) DNA of Escherichia coli in the chromosomal DNA of wild-type C. freundii. Protons, but not sodium ions, were found to be the coupling cations for melibiose (and methyl-beta -D-thiogalactoside) transport in the mutant cells.

Copyright © 1998 by American Society for Microbiology




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