This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Valenzuela, L.
Right arrow Articles by González, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Valenzuela, L.
Right arrow Articles by González, A.

 Previous Article  |  Next Article 

J Bacteriol, July 1998, p. 3533-3540, Vol. 180, No. 14
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Regulation of Expression of GLT1, the Gene Encoding Glutamate Synthase in Saccharomyces cerevisiae

Lourdes Valenzuela,1 Paola Ballario,2 Cristina Aranda,1 Patrizia Filetici,2 and Alicia González1,*

Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico,1 and Dipartimento di Genetica e Biologia Molecolare, Centro de Studio per gli Acidi Nucleici, Universitá "La Sapienza," 00185 Rome, Italy2

Received 12 March 1998/Accepted 12 May 1998

Saccharomyces cerevisiae glutamate synthase (GOGAT) is an oligomeric enzyme composed of three 199-kDa identical subunits encoded by GLT1. In this work, we analyzed GLT1 transcriptional regulation. GLT1-lacZ fusions were prepared and GLT1 expression was determined in a GDH1 wild-type strain and in a gdh1 mutant derivative grown in the presence of various nitrogen sources. Null mutants impaired in GCN4, GLN3, GAT1/NIL1, or UGA43/DAL80 were transformed with a GLT1-lacZ fusion to determine whether the above-mentioned transcriptional factors had a role in GLT1 expression. A collection of increasingly larger 5' deletion derivatives of the GLT1 promoter was constructed to identify DNA sequences that could be involved in GLT1 transcriptional regulation. The effect of the lack of GCN4, GLN3, or GAT1/NIL1 was also tested in the pertinent 5' deletion derivatives. Our results indicate that (i) GLT1 expression is negatively modulated by glutamate-mediated repression and positively regulated by Gln3p- and Gcn4p-dependent transcriptional activation; (ii) two cis-acting elements, a CGGN15CCG palindrome and an imperfect poly(dA-dT), are present and could play a role in GLT1 transcriptional activation; and (iii) GLT1 expression is moderately regulated by GCN4 under amino acid deprivation. Our results suggest that in a wild-type strain grown on ammonium, GOGAT constitutes an ancillary pathway for glutamate biosynthesis.

* Corresponding author. Mailing address: Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Apartado Postal 70-242, Mexico City 04510, Mexico. Phone: 6225631. Fax: 6225630. E-mail: amanjarr{at}ifisiol.unam.mx.

J Bacteriol, July 1998, p. 3533-3540, Vol. 180, No. 14
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kim, J., Kim, H. (2008). Clustering of change patterns using Fourier coefficients. Bioinformatics 24: 184-191 [Abstract] [Full Text]  
  • Godard, P., Urrestarazu, A., Vissers, S., Kontos, K., Bontempi, G., van Helden, J., Andre, B. (2007). Effect of 21 Different Nitrogen Sources on Global Gene Expression in the Yeast Saccharomyces cerevisiae. Mol. Cell. Biol. 27: 3065-3086 [Abstract] [Full Text]  
  • Shakoury-Elizeh, M., Tiedeman, J., Rashford, J., Ferea, T., Demeter, J., Garcia, E., Rolfes, R., Brown, P. O., Botstein, D., Philpott, C. C. (2004). Transcriptional Remodeling in Response to Iron Deprivation in Saccharomyces cerevisiae. Mol. Biol. Cell 15: 1233-1243 [Abstract] [Full Text]  
  • DeLuna, A., Avendano, A., Riego, L., Gonzalez, A. (2001). NADP-Glutamate Dehydrogenase Isoenzymes of Saccharomyces cerevisiae. PURIFICATION, KINETIC PROPERTIES, AND PHYSIOLOGICAL ROLES. J. Biol. Chem. 276: 43775-43783 [Abstract] [Full Text]  
  • Natarajan, K., Meyer, M. R., Jackson, B. M., Slade, D., Roberts, C., Hinnebusch, A. G., Marton, M. J. (2001). Transcriptional Profiling Shows that Gcn4p Is a Master Regulator of Gene Expression during Amino Acid Starvation in Yeast. Mol. Cell. Biol. 21: 4347-4368 [Abstract] [Full Text]  
  • Valenzuela, L., Aranda, C., González, A. (2001). TOR Modulates GCN4-Dependent Expression of Genes Turned on by Nitrogen Limitation. J. Bacteriol. 183: 2331-2334 [Abstract] [Full Text]  
  • Romero, M., Guzmán-León, S., Aranda, C., González-Halphen, D., Valenzuela, L., González, A. (2000). Pathways for glutamate biosynthesis in the yeast Kluyveromyces lactis. Microbiology 146: 239-245 [Abstract] [Full Text]  
  • Palmieri, L., Agrimi, G., Runswick, M. J., Fearnley, I. M., Palmieri, F., Walker, J. E. (2001). Identification in Saccharomyces cerevisiae of Two Isoforms of a Novel Mitochondrial Transporter for 2-Oxoadipate and 2-Oxoglutarate. J. Biol. Chem. 276: 1916-1922 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»