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J Bacteriol, July 1998, p. 3727-3729, Vol. 180, No. 14
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Minisatellite Sequence within the Propeptide Region of the Vacuolar Carboxypeptidase Y Gene of Schizosaccharomyces pombe

Susham S. Ingavale, Rupinder Kaur, Parul Aggarwal, and Anand K. Bachhawat*

Institute of Microbial Technology, Chandigarh---160 036, India

Received 11 March 1998/Accepted 7 May 1998

We describe the presence of a minisatellite sequence that displays length polymorphisms in the fission yeast Schizosaccharomyces pombe. The minisatellite sequence was found to reside within the propeptide region of the vacuolar carboxypeptidase Y gene. The minisatellite sequence, which was found only at a single locus, was mitotically stable and displayed length polymorphisms between the two varieties of S. pombe (S. pombe var. pombe and S. pombe var. malidevorans). The minisatellite sequence, however, appeared to be species specific and was absent in other members of the Schizosaccharomyces genus. This report constitutes the first experimental demonstration of the presence of such sequences in yeasts.

* Corresponding author. Mailing address: Institute of Microbial Technology, Sector 39-A, Chandigarh---160 036, India. Phone: 0172-690004. Fax: 0172-690585 or 0172-690632. E-mail: abachhawat{at}usa.net.

J Bacteriol, July 1998, p. 3727-3729, Vol. 180, No. 14
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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