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Journal of Bacteriology, August 1998, p. 3785-3792, Vol. 180, No. 15
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Biosynthesis of Di-myo-Inositol-1,1'-Phosphate, a Novel Osmolyte in Hyperthermophilic Archaea

Liangjing Chen, Elias T. Spiliotis, and Mary F. Roberts*

Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02167

Received 27 March 1997/Accepted 26 May 1998

Biosynthesis of di-myo-inositol-1,1'-phosphate (DIP) is proposed to occur with myo-inositol and myo-inositol 1-phosphate (I-1-P) used as precursors. Activation of the I-1-P with CTP and condensation of the resultant CDP-inositol (CDP-I) with myo-inositol then generates DIP. The sole known biosynthetic pathway of inositol in all organisms is the conversion of D-glucose-6-phosphate to myo-inositol. This conversion requires two key enzymes: L-I-1-P synthase and I-1-P phosphatase. Enzymatic assays using 31P nuclear magnetic resonance spectroscopy as well as a colorimetric assay for inorganic phosphate have confirmed the occurrence of L-I-1-P synthase and a moderately specific I-1-P phosphatase. The enzymatic reaction that couples CDP-I with myo-inositol to generate DIP has also been detected in Methanococcus igneus. 13C labeling studies with [2,3-13C]pyruvate and [3-13C]pyruvate were used to examine this pathway in M. igneus. Label distribution in DIP was consistent with inositol units formed from glucose-6-phosphate, but the label in the glucose moiety was scrambled via transketolase and transaldolase activities of the pentose phosphate pathway.

* Corresponding author. Merkert Chemistry Center, Boston College, 2609 Beacon St., Chestnut Hill, MA 02167. Phone: (617) 552-3616. Fax: (617) 552-2705. E-mail: mary.roberts{at}bc.edu.

Journal of Bacteriology, August 1998, p. 3785-3792, Vol. 180, No. 15
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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