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Journal of Bacteriology, August 1998, p. 3882-3890, Vol. 180, No. 15
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

YscO of Yersinia pestis Is a Mobile Core Component of the Yop Secretion System

Patricia L. Payne and Susan C. Straley*

Department of Microbiology and Immunology, Albert B. Chandler Medical Center, University of Kentucky, Lexington, Kentucky 40536-0084

Received 21 January 1998/Accepted 22 May 1998

The Yersinia pestis low-Ca2+ response stimulon is responsible for the temperature- and Ca2+-regulated expression and secretion of plasmid pCD1-encoded antihost proteins (V antigen and Yops). We have previously shown that lcrD, yscC, yscD, yscG, and yscR encode proteins that are essential for high-level expression and secretion of V antigen and Yops at 37°C in the absence of Ca2+. In this study, we characterized yscO of the Yop secretion (ysc) operon that contains yscN through yscU by determining the localization of its gene product and the phenotype of an in-frame deletion. The yscO mutant grew and expressed the same levels of Yops as the parent at 37°C in the presence of Ca2+. In the absence of Ca2+, the mutant grew independently of Ca2+, expressed only basal levels of V antigen and Yops, and failed to secrete these. These defects could be partially complemented by providing yscO in trans in the yscO mutant. Overexpression of YopM and V antigen in the mutant failed to restore the export of either protein, showing that the mutation had a direct effect on secretion. These results indicated that the yscO gene product is required for high-level expression and secretion of V antigen and Yops. YscO was found by immunoblot analysis in the soluble and membrane fractions of bacteria growing at 37°C irrespective of the presence of Ca2+ and in the culture medium in the absence of Ca2+. YscO is the only mobile protein identified so far in the Yersinia species that is required for secretion of V antigen and Yops.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Albert B. Chandler Medical Center, University of Kentucky, Lexington, KY 40536-0084. Phone: (606) 323-6538. Fax: (606) 257-8994. E-mail: scstra01{at}pop.uky.edu.

Journal of Bacteriology, August 1998, p. 3882-3890, Vol. 180, No. 15
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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