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Journal of Bacteriology, August 1998, p. 3946-3953, Vol. 180, No. 15
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Bacterial SOS Checkpoint Protein SulA Inhibits Polymerization of Purified FtsZ Cell Division Protein

Dorina Trusca,1 Solomon Scott,2 Chris Thompson,1 and David Bramhill1,*

Department of Enzymology1 and Department of Immunology,2 Merck Research Laboratories, Rahway, New Jersey 07065-0900

Received 6 February 1998/Accepted 29 May 1998

Cell division of Escherichia coli is inhibited when the SulA protein is induced in response to DNA damage as part of the SOS checkpoint control system. The SulA protein interacts with the tubulin-like FtsZ division protein. We investigated the effects of purified SulA upon FtsZ. SulA protein inhibits the polymerization and the GTPase activity of FtsZ, while point mutant SulA proteins show little effect on either of these FtsZ activities. SulA did not inhibit the polymerization of purified FtsZ2 mutant protein, which was originally isolated as insensitive to SulA. These studies define polymerization assays for FtsZ which respond to an authentic cellular regulator. The observations presented here support the notion that polymerization of FtsZ is central to its cellular role and that direct, reversible inhibition of FtsZ polymerization by SulA may account for division inhibition.

* Corresponding author. Mailing address: Department of Enzymology, Building 80Y-325, Merck Research Laboratories, 126 East Lincoln Avenue, Rahway, NJ 07065-0900. Phone: (732) 594-6092. Fax: (732) 594-5878. E-mail: david_bramhill{at}merck.com.

Journal of Bacteriology, August 1998, p. 3946-3953, Vol. 180, No. 15
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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