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Journal of Bacteriology, August 1998, p. 4030-4035, Vol. 180, No. 16
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Molecular Cloning and Functional Expression of a Protein-Serine/Threonine Phosphatase from the Hyperthermophilic Archaeon Pyrodictium abyssi TAG11

Bianca Mai,1 Gerhard Frey,1,* Ronald V. Swanson,2 Eric J. Mathur,2 and K. O. Stetter1

Lehrstuhl für Mikrobiologie, Universität Regensburg, 93053 Regensburg, Germany,1 and Diversa Corp., San Diego, California 921212

Received 17 November 1997/Accepted 3 June 1998

An open reading frame coding for a putative protein-serine/threonine phosphatase was identified in the hyperthermophilic archaeon Pyrodictium abyssi TAG11 and named Py-PP1. Py-PP1 was expressed in Escherichia coli, purified from inclusion bodies, and biochemically characterized. The phosphatase gene is part of an operon which may provide, for the first time, insight into a physiological role for archaeal protein phosphatases in vivo.

* Corresponding author. Mailing address: Lehrstuhl für Mikrobiologie, Universitätsstr. 31, D-93053 Regensburg, Germany. Phone: 0941/943-3178. Fax: 0941/943-2403. E-mail: Gerhard.Frey{at}biologie.uni-regensburg.de.

Journal of Bacteriology, August 1998, p. 4030-4035, Vol. 180, No. 16
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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