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Journal of Bacteriology, August 1998, p. 4270-4277, Vol. 180, No. 16
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Analysis of the puc Operon Promoter from Rhodobacter capsulatus

David G. Nickens and Carl E. Bauer*

Department of Biology, Indiana University, Bloomington, Indiana 47405

Received 8 April 1998/Accepted 9 June 1998

Expression of the Rhodobacter capsulatus puc operon, which codes for structural polypeptides of the light-harvesting-II peripheral antenna complex, is highly regulated in response to alterations in oxygen tension and light intensity. To obtain an understanding of the puc promoter region we report the high-resolution 5' mapping of the puc mRNA transcriptional start site and DNA sequence analysis of the puc upstream regulatory sequence (pucURS). A sigma 70-type promoter sequence was identified (pucP1) which has a high degree of sequence similarity with carotenoid and bacteriochlorophyll biosynthesis promoters. Inspection of the DNA sequence also indicated the presence of two CrtJ and four integration host factor (IHF) binding sites. Transcriptional fusions of the pucURS fused to lacZ also confirmed that puc promoter activity is regulated by the transcriptional regulators IHF, CrtJ, and RegA. Gel retardation analysis using cell extracts indicates that mutations in IHF and RegA disrupt protein binding to DNA fragments containing the pucURS.

* Corresponding author. Mailing address: Department of Biology, Jordan Hall, Indiana University, Bloomington, IN 47405. Phone: (812) 855-6595. Fax: (812) 855-6705. E-mail: cbauer{at}bio.indiana.edu.

Journal of Bacteriology, August 1998, p. 4270-4277, Vol. 180, No. 16
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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