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Journal of Bacteriology, August 1998, p. 4300-4302, Vol. 180, No. 16
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Agrobacterium VirE2 Proteins Can Form a Complex with T Strands in the Plant Cytoplasm

Stanton B. Gelvin*

Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392

Received 7 April 1998/Accepted 4 June 1998

Wild-type VirE2 and VirD2 proteins from Agrobacterium tumefaciens contain nuclear targeting sequences (NLS) that are likely involved in directing transferred T strands to the plant nucleus. An A. tumefaciens virE2 virD2Delta NLS double mutant was able to form tumors on VirE2-producing transgenic tobacco but not on wild-type tobacco. Because this mutant bacterial strain contains no known T-strand nuclear targeting signal, the data indicate that wild-type VirE2 proteins produced by the plant can interact with the T strands in the plant cytoplasm and direct them to the nucleus.

* Mailing address: Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-1392. Phone: (765) 494-4939. Fax: (765) 496-1496. E-mail: gelvin{at}bilbo.bio.purdue.edu.

Journal of Bacteriology, August 1998, p. 4300-4302, Vol. 180, No. 16
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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