This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fujita, Y.
Right arrow Articles by Kasahara, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fujita, Y.
Right arrow Articles by Kasahara, Y.

 Previous Article  |  Next Article 

Journal of Bacteriology, August 1998, p. 4309-4313, Vol. 180, No. 16
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Identification and Expression of the Bacillus subtilis Fructose-1,6-Bisphosphatase Gene (fbp)

Yasutaro Fujita,1,* Ken-Ichi Yoshida,1 Yasuhiko Miwa,1 Nobuo Yanai,1 Eishi Nagakawa,1 and Yasuhiro Kasahara2

Department of Biotechnology, Faculty of Engineering, Fukuyama University, Fukuyama 729-0292,1 and Graduate School of Biological Sciences, Nara Advanced Institute of Science and Technology, Ikoma 630-0101,2 Japan

Received 12 March 1998/Accepted 9 June 1998

The Bacillus subtilis fbp gene encoding fructose-1,6-bisphosphatase (FBPase) was originally identified as yydE. The fbp gene was expressed at a fairly constant level in cells undergoing glycolysis or gluconeogenesis. fbp transcription was initiated 94 bp upstream of the translation initiation codon, resulting in a 2.4-kb monocistronic transcript. Interestingly, B. subtilis FBPase exhibited no significant similarity to other FBPases in protein sequence databases.

* Corresponding author. Mailing address: Department of Biotechnology, Faculty of Engineering, Fukuyama University, 985 Sanzo, Higashimura-cho, Fukuyama-shi, Hiroshima 729-0292, Japan. Phone: (81)849 36 2111. Fax: (81)849 36 2023. E-mail: yfujita{at}bt.fubt.fukuyama-u.ac.jp.

Journal of Bacteriology, August 1998, p. 4309-4313, Vol. 180, No. 16
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Jules, M., Le Chat, L., Aymerich, S., Le Coq, D. (2009). The Bacillus subtilis ywjI (glpX) Gene Encodes a Class II Fructose-1,6-Bisphosphatase, Functionally Equivalent to the Class III Fbp Enzyme. J. Bacteriol. 191: 3168-3171 [Abstract] [Full Text]  
  • Kazimierczak, K. A., Scott, K. P., Kelly, D., Aminov, R. I. (2009). Tetracycline Resistome of the Organic Pig Gut. Appl. Environ. Microbiol. 75: 1717-1722 [Abstract] [Full Text]  
  • Brown, G., Singer, A., Lunin, V. V., Proudfoot, M., Skarina, T., Flick, R., Kochinyan, S., Sanishvili, R., Joachimiak, A., Edwards, A. M., Savchenko, A., Yakunin, A. F. (2009). Structural and Biochemical Characterization of the Type II Fructose-1,6-bisphosphatase GlpX from Escherichia coli. J. Biol. Chem. 284: 3784-3792 [Abstract] [Full Text]  
  • Yoshida, K.-i., Yamaguchi, M., Morinaga, T., Kinehara, M., Ikeuchi, M., Ashida, H., Fujita, Y. (2008). myo-Inositol Catabolism in Bacillus subtilis. J. Biol. Chem. 283: 10415-10424 [Abstract] [Full Text]  
  • Hines, J. K., Fromm, H. J., Honzatko, R. B. (2007). Structures of Activated Fructose-1,6-bisphosphatase from Escherichia coli: COORDINATE REGULATION OF BACTERIAL METABOLISM AND THE CONSERVATION OF THE R-STATE. J. Biol. Chem. 282: 11696-11704 [Abstract] [Full Text]  
  • Bertram, R., Kostner, M., Muller, J., Ramos, J. V., Hillen, W. (2005). Integrative elements for Bacillus subtilis yielding tetracycline-dependent growth phenotypes. Nucleic Acids Res 33: e153-e153 [Abstract] [Full Text]  
  • Movahedzadeh, F., Rison, S. C. G., Wheeler, P. R., Kendall, S. L., Larson, T. J., Stoker, N. G. (2004). The Mycobacterium tuberculosis Rv1099c gene encodes a GlpX-like class II fructose 1,6-bisphosphatase. Microbiology 150: 3499-3505 [Abstract] [Full Text]  
  • Sato, T., Imanaka, H., Rashid, N., Fukui, T., Atomi, H., Imanaka, T. (2004). Genetic Evidence Identifying the True Gluconeogenic Fructose-1,6-Bisphosphatase in Thermococcus kodakaraensis and Other Hyperthermophiles. J. Bacteriol. 186: 5799-5807 [Abstract] [Full Text]  
  • Yoshida, K.-i., Yamaguchi, M., Ikeda, H., Omae, K., Tsurusaki, K.-i., Fujita, Y. (2004). The fifth gene of the iol operon of Bacillus subtilis, iolE, encodes 2-keto-myo-inositol dehydratase. Microbiology 150: 571-580 [Abstract] [Full Text]  
  • Rashid, N., Imanaka, H., Kanai, T., Fukui, T., Atomi, H., Imanaka, T. (2002). A Novel Candidate for the True Fructose-1,6-bisphosphatase in Archaea. J. Biol. Chem. 277: 30649-30655 [Abstract] [Full Text]  
  • Verhees, C. H., Akerboom, J., Schiltz, E., de Vos, W. M., van der Oost, J. (2002). Molecular and Biochemical Characterization of a Distinct Type of Fructose-1,6-Bisphosphatase from Pyrococcus furiosus. J. Bacteriol. 184: 3401-3405 [Abstract] [Full Text]  
  • Chetouani, F., Glaser, P., Kunst, F. (2001). FindTarget: software for subtractive genome analysis. Microbiology 147: 2643-2649 [Abstract] [Full Text]  
  • Chalker, A. F., Minehart, H. W., Hughes, N. J., Koretke, K. K., Lonetto, M. A., Brinkman, K. K., Warren, P. V., Lupas, A., Stanhope, M. J., Brown, J. R., Hoffman, P. S. (2001). Systematic Identification of Selective Essential Genes in Helicobacter pylori by Genome Prioritization and Allelic Replacement Mutagenesis. J. Bacteriol. 183: 1259-1268 [Abstract] [Full Text]  
  • Yoshida, K.-i., Kobayashi, K., Miwa, Y., Kang, C.-M., Matsunaga, M., Yamaguchi, H., Tojo, S., Yamamoto, M., Nishi, R., Ogasawara, N., Nakayama, T., Fujita, Y. (2001). Combined transcriptome and proteome analysis as a powerful approach to study genes under glucose repression in Bacillus subtilis. Nucleic Acids Res 29: 683-692 [Abstract] [Full Text]  
  • Yoshida, K.-I., Fujita, Y., Ehrlich, S. D. (2000). An Operon for a Putative ATP-Binding Cassette Transport System Involved in Acetoin Utilization of Bacillus subtilis. J. Bacteriol. 182: 5454-5461 [Abstract] [Full Text]  
  • Donahue, J. L., Bownas, J. L., Niehaus, W. G., Larson, T. J. (2000). Purification and Characterization of glpX-Encoded Fructose 1,6-Bisphosphatase, a New Enzyme of the Glycerol 3-Phosphate Regulon of Escherichia coli. J. Bacteriol. 182: 5624-5627 [Abstract] [Full Text]  
  • Yoshida, K.-i., Ishio, I., Nagakawa, E., Yamamoto, Y., Yamamoto, M., Fujita, Y. (2000). Systematic study of gene expression and transcription organization in the gntZ-ywaA region of the Bacillus subtilis genome. Microbiology 146: 573-579 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»