This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Berg, T.
Right arrow Articles by Skurray, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Berg, T.
Right arrow Articles by Skurray, R. A.

 Previous Article  |  Next Article 

Journal of Bacteriology, September 1998, p. 4350-4359, Vol. 180, No. 17
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Complete Nucleotide Sequence of pSK41: Evolution of Staphylococcal Conjugative Multiresistance Plasmids

Tracey Berg, Neville Firth, Sumalee Apisiridej,dagger Anusha Hettiaratchi, Amornrut Leelaporn,Dagger and Ronald A. Skurray*

School of Biological Sciences, University of Sydney, New South Wales 2006, Australia

Received 5 May 1998/Accepted 16 June 1998

The 46.4-kb nucleotide sequence of pSK41, a prototypical multiresistance plasmid from Staphylococcus aureus, has been determined, representing the first completely sequenced conjugative plasmid from a gram-positive organism. Analysis of the sequence has enabled the identification of the probable replication, maintenance, and transfer functions of the plasmid and has provided insights into the evolution of a clinically significant group of plasmids. The basis of deletions commonly associated with pSK41 family plasmids has been investigated, as has the observed insertion site specificity of Tn552-like beta -lactamase transposons within them. Several of the resistance determinants carried by pSK41-like plasmids were found to be located on up to four smaller cointegrated plasmids. pSK41 and related plasmids appear to represent a consolidation of antimicrobial resistance functions, collected by a preexisting conjugative plasmid via transposon insertion and IS257-mediated cointegrative capture of other plasmids.

* Corresponding author. Mailing address: School of Biological Sciences, University of Sydney, Sydney, New South Wales 2006, Australia. Phone: 61-2-9351-2376. Fax: 61-2-9351-4771. E-mail: skurray{at}bio.usyd.edu.au.

dagger Present address: Division of Medical Microbiology, Department of Pathology, Faculty of Medicine, Prince of Songkla University, Hadyai 90112, Thailand.

Dagger Present address: Department of Microbiology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.

Journal of Bacteriology, September 1998, p. 4350-4359, Vol. 180, No. 17
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ni, L., Jensen, S. O., Ky Tonthat, N., Berg, T., Kwong, S. M., Guan, F. H. X., Brown, M. H., Skurray, R. A., Firth, N., Schumacher, M. A. (2009). The Staphylococcus aureus pSK41 plasmid-encoded ArtA protein is a master regulator of plasmid transmission genes and contains a RHH motif used in alternate DNA-binding modes. Nucleic Acids Res 0: gkp756v1-gkp756 [Abstract] [Full Text]  
  • Zhu, L.-X., Zhang, Z.-W., Wang, C., Yang, H.-W., Jiang, D., Zhang, Q., Mitchelson, K., Cheng, J. (2007). Use of a DNA Microarray for Simultaneous Detection of Antibiotic Resistance Genes among Staphylococcal Clinical Isolates. J. Clin. Microbiol. 45: 3514-3521 [Abstract] [Full Text]  
  • Perez-Roth, E., Lopez-Aguilar, C., Alcoba-Florez, J., Mendez-Alvarez, S. (2006). High-Level Mupirocin Resistance within Methicillin-Resistant Staphylococcus aureus Pandemic Lineages.. Antimicrob. Agents Chemother. 50: 3207-3211 [Abstract] [Full Text]  
  • Bannam, T. L., Teng, W. L., Bulach, D., Lyras, D., Rood, J. I. (2006). Functional Identification of Conjugation and Replication Regions of the Tetracycline Resistance Plasmid pCW3 from Clostridium perfringens. J. Bacteriol. 188: 4942-4951 [Abstract] [Full Text]  
  • Francia, M. V., Fujimoto, S., Tille, P., Weaver, K. E., Clewell, D. B. (2004). Replication of Enterococcus faecalis Pheromone-Responding Plasmid pAD1: Location of the Minimal Replicon and oriV Site and RepA Involvement in Initiation of Replication. J. Bacteriol. 186: 5003-5016 [Abstract] [Full Text]  
  • Sherley, M., Gordon, D. M., Collignon, P. J. (2004). Evolution of multi-resistance plasmids in Australian clinical isolates of Escherichia coli. Microbiology 150: 1539-1546 [Abstract] [Full Text]  
  • Flannagan, S. E., Chow, J. W., Donabedian, S. M., Brown, W. J., Perri, M. B., Zervos, M. J., Ozawa, Y., Clewell, D. B. (2003). Plasmid Content of a Vancomycin-Resistant Enterococcus faecalis Isolate from a Patient Also Colonized by Staphylococcus aureus with a VanA Phenotype. Antimicrob. Agents Chemother. 47: 3954-3959 [Abstract] [Full Text]  
  • Weigel, L. M., Clewell, D. B., Gill, S. R., Clark, N. C., McDougal, L. K., Flannagan, S. E., Kolonay, J. F., Shetty, J., Killgore, G. E., Tenover, F. C. (2003). Genetic Analysis of a High-Level Vancomycin-Resistant Isolate of Staphylococcus aureus. Science 302: 1569-1571 [Abstract] [Full Text]  
  • Vakulenko, S. B., Mobashery, S. (2003). Versatility of Aminoglycosides and Prospects for Their Future. Clin. Microbiol. Rev. 16: 430-450 [Abstract] [Full Text]  
  • Koning, S., van Belkum, A., Snijders, S., van Leeuwen, W., Verbrugh, H., Nouwen, J., Op 't Veld, M., van Suijlekom-Smit, L. W. A., van der Wouden, J. C., Verduin, C. (2003). Severity of Nonbullous Staphylococcus aureus Impetigo in Children Is Associated with Strains Harboring Genetic Markers for Exfoliative Toxin B, Panton-Valentine Leukocidin, and the Multidrug Resistance Plasmid pSK41. J. Clin. Microbiol. 41: 3017-3021 [Abstract] [Full Text]  
  • Grohmann, E., Muth, G., Espinosa, M. (2003). Conjugative Plasmid Transfer in Gram-Positive Bacteria. Microbiol. Mol. Biol. Rev. 67: 277-301 [Abstract] [Full Text]  
  • Lange, C. C., Werckenthin, C., Schwarz, S. (2003). Molecular analysis of the plasmid-borne aacA/aphD resistance gene region of coagulase-negative staphylococci from chickens. J Antimicrob Chemother 51: 1397-1401 [Abstract] [Full Text]  
  • Simpson, A. E., Skurray, R. A., Firth, N. (2003). A Single Gene on the Staphylococcal Multiresistance Plasmid pSK1 Encodes a Novel Partitioning System. J. Bacteriol. 185: 2143-2152 [Abstract] [Full Text]  
  • Sanchez-Hidalgo, M., Maqueda, M., Galvez, A., Abriouel, H., Valdivia, E., Martinez-Bueno, M. (2003). The Genes Coding for Enterocin EJ97 Production by Enterococcus faecalis EJ97 Are Located on a Conjugative Plasmid. Appl. Environ. Microbiol. 69: 1633-1641 [Abstract] [Full Text]  
  • Grkovic, S., Brown, M. H., Hardie, K. M., Firth, N., Skurray, R. A. (2003). Stable low-copy-number Staphylococcus aureus shuttle vectors. Microbiology 149: 785-794 [Abstract] [Full Text]  
  • Inui, M., Nakata, K., Roh, J. H., Vertes, A. A., Yukawa, H. (2003). Isolation and Molecular Characterization of pMG160, a Mobilizable Cryptic Plasmid from Rhodobacter blasticus. Appl. Environ. Microbiol. 69: 725-733 [Abstract] [Full Text]  
  • Calcutt, M. J., Lewis, M. S., Wise, K. S. (2002). Molecular Genetic Analysis of ICEF, an Integrative Conjugal Element That Is Present as a Repetitive Sequence in the Chromosome of Mycoplasma fermentans PG18. J. Bacteriol. 184: 6929-6941 [Abstract] [Full Text]  
  • Anthonisen, I.-L., Sunde, M., Steinum, T. M., Sidhu, M. S., Sorum, H. (2002). Organization of the Antiseptic Resistance Gene qacA and Tn552-Related {beta}-Lactamase Genes in Multidrug- Resistant Staphylococcus haemolyticus Strains of Animal and Human Origins. Antimicrob. Agents Chemother. 46: 3606-3612 [Abstract] [Full Text]  
  • Sidhu, M. S., Heir, E., Leegaard, T., Wiger, K., Holck, A. (2002). Frequency of Disinfectant Resistance Genes and Genetic Linkage with {beta}-Lactamase Transposon Tn552 among Clinical Staphylococci. Antimicrob. Agents Chemother. 46: 2797-2803 [Abstract] [Full Text]  
  • Ida, T., Okamoto, R., Nonoyama, M., Irinoda, K., Kurazono, M., Inoue, M. (2002). Antagonism between Aminoglycosides and {beta}-Lactams in a Methicillin-Resistant Staphylococcus aureus Isolate Involves Induction of an Aminoglycoside-Modifying Enzyme. Antimicrob. Agents Chemother. 46: 1516-1521 [Abstract] [Full Text]  
  • Kurenbach, B., Grothe, D., Farias, M. E., Szewzyk, U., Grohmann, E. (2002). The tra Region of the Conjugative Plasmid pIP501 Is Organized in an Operon with the First Gene Encoding the Relaxase. J. Bacteriol. 184: 1801-1805 [Abstract] [Full Text]  
  • Yamaguchi, T., Hayashi, T., Takami, H., Ohnishi, M., Murata, T., Nakayama, K., Asakawa, K., Ohara, M., Komatsuzawa, H., Sugai, M. (2001). Complete Nucleotide Sequence of a Staphylococcus aureus Exfoliative Toxin B Plasmid and Identification of a Novel ADP-Ribosyltransferase, EDIN-C. Infect. Immun. 69: 7760-7771 [Abstract] [Full Text]  
  • Bjorland, J., Sunde, M., Waage, S. (2001). Plasmid-Borne smr Gene Causes Resistance to Quaternary Ammonium Compounds in Bovine Staphylococcus aureus. J. Clin. Microbiol. 39: 3999-4004 [Abstract] [Full Text]  
  • Martinez, B., Tomkins, J., Wackett, L. P., Wing, R., Sadowsky, M. J. (2001). Complete Nucleotide Sequence and Organization of the Atrazine Catabolic Plasmid pADP-1 from Pseudomonas sp. Strain ADP. J. Bacteriol. 183: 5684-5697 [Abstract] [Full Text]  
  • Hickey, R. M., Twomey, D. P., Ross, R. P., Hill, C. (2001). Exploitation of Plasmid pMRC01 To Direct Transfer of Mobilizable Plasmids into Commercial Lactococcal Starter Strains. Appl. Environ. Microbiol. 67: 2853-2858 [Abstract] [Full Text]  
  • Garnier, F., Taourit, S., Glaser, P., Courvalin, P., Galimand, M. (2000). Characterization of transposon Tn1549, conferring VanB-type resistance in Enterococcus spp.. Microbiology 146: 1481-1489 [Abstract] [Full Text]  
  • Firth, N., Apisiridej, S., Berg, T., O'Rourke, B. A., Curnock, S., Dyke, K. G. H., Skurray, R. A. (2000). Replication of Staphylococcal Multiresistance Plasmids. J. Bacteriol. 182: 2170-2178 [Abstract] [Full Text]  
  • Haake, S. K., Yoder, S. C., Attarian, G., Podkaminer, K. (2000). Native Plasmids of Fusobacterium nucleatum: Characterization and Use in Development of Genetic Systems. J. Bacteriol. 182: 1176-1180 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»