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Journal of Bacteriology, September 1998, p. 4413-4415, Vol. 180, No. 17
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Denitrification by Actinomycetes and Purification of Dissimilatory Nitrite Reductase and Azurin from Streptomyces thioluteus

Hirofumi Shoun,1,2,* Mitsuyoshi Kano,1 Ikuko Baba,1 Naoki Takaya,1 and Masaru Matsuo1

Institute of Applied Biochemistry1 and Center for Tsukuba Advanced Research Alliance (TARA),2 University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan

Received 12 February 1998/Accepted 25 June 1998

Many actinomycete strains are able to convert nitrate or nitrite to nitrous oxide (N2O). As a representative of actinomycete denitrification systems, the system of Streptomyces thioluteus was investigated in detail. S. thioluteus attained distinct cell growth upon anaerobic incubation with nitrate or nitrite with concomitant and stoichiometric conversion of nitrate or nitrite to N2O, suggesting that the denitrification acts as anaerobic respiration. Furthermore, a copper-containing, dissimilatory nitrite reductase (CuNir) and its physiological electron donor, azurin, were isolated. This is the first report to show that denitrification generally occurs among actinomycetes.

* Corresponding author. Mailing address: Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan. Phone: 81 298 53 4603. Fax: 81 298 53 4605. E-mail: p450nor{at}sakura.cc.tsukuba.ac.jp.

Journal of Bacteriology, September 1998, p. 4413-4415, Vol. 180, No. 17
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kumon, Y., Sasaki, Y., Kato, I., Takaya, N., Shoun, H., Beppu, T. (2002). Codenitrification and Denitrification Are Dual Metabolic Pathways through Which Dinitrogen Evolves from Nitrate in Streptomyces antibioticus. J. Bacteriol. 184: 2963-2968 [Abstract] [Full Text]  
  • Moroz, L. L. (2001). Gaseous Transmission Across Time and Species. Integr. Comp. Biol. 41: 304-320 [Abstract] [Full Text]  


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