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Journal of Bacteriology, September 1998, p. 4508-4515, Vol. 180, No. 17
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Transcriptional Regulation of CLN3 Expression by Glucose in Saccharomyces cerevisiae

Fereshteh Parviz,1 Duane D. Hall,2 David D. Markwardt,1 and Warren Heideman1,2,*

School of Pharmacy1 and Department of Biomolecular Chemistry,2 University of Wisconsin, Madison, Wisconsin

Received 18 May 1998/Accepted 25 June 1998

In Saccharomyces cerevisiae, the transition from the G1 phase of the mitotic cycle into S phase is controlled by a set of G1 cyclins that regulate the activity of the protein kinase encoded by CDC28. Yeast cells regulate progress through the G1/S boundary in response to nutrients, moving quickly through G1 in glucose medium and more slowly in poorer medium. We have examined connections between glucose and the level of the message encoding Cln3, a G1 cyclin. We found that glucose positively regulates CLN3 mRNA levels through a set of repeated AAGAAAAA (A2GA5) elements within the CLN3 promoter. Mutations in these sequences reduce both transcriptional activation and specific interaction between CLN3 promoter elements and proteins in yeast extracts. Creation of five point mutations, replacing the G's within these repeats with T's, in the CLN3 promoter substantially reduces CLN3 expression in glucose medium and inhibits the ability of the cells to maintain a constant size when shifted into glucose.

* Corresponding author. Mailing address: School of Pharmacy, University of Wisconsin, 425 N. Charter Street, Madison, WI 53706. Phone: (608) 262-1795. Fax: (608) 262 3397. E-mail: wheidema{at}facstaff.wisc.edu.

Journal of Bacteriology, September 1998, p. 4508-4515, Vol. 180, No. 17
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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