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Journal of Bacteriology, September 1998, p. 4739-4741, Vol. 180, No. 17
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Salmonella typhimurium nit Is nadE: Defective Nitrogen Utilization and Ammonia-Dependent NAD Synthetase

Barbara L. Schneiderdagger and Lawrence J. Reitzer*

Department of Molecular and Cell Biology, The University of Texas at Dallas, Richardson, Texas, 75083-0688

Received 2 March 1998/Accepted 20 June 1998

S. typhimurium nit mutants are defective in nitrogen assimilation, despite having normal levels of assimilatory enzymes. Complementation, enzyme assays, and genetic mapping show that nit is nadE. We present evidence that ammonia, not glutamine, is the physiological substrate for eubacterial NAD synthetases and that low activity completely accounts for the mutant phenotype.

* Corresponding author. Mailing address: Department of Molecular and Cell Biology, Mail Station FO 3.1, The University of Texas at Dallas, P. O. Box 830688, Richardson, TX 75083-0688. Phone: (972) 883-2523. Fax: (972) 883-2409. E-mail: reitzer{at}utdallas.edu.

dagger Present address: Department of Biology, University of California at San Diego, La Jolla, CA 92093-0116.

Journal of Bacteriology, September 1998, p. 4739-4741, Vol. 180, No. 17
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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