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Journal of Bacteriology, September 1998, p. 4753-4756, Vol. 180, No. 17
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The pcd Gene Encoding Piperideine-6-Carboxylate Dehydrogenase Involved in Biosynthesis of alpha -Aminoadipic Acid Is Located in the Cephamycin Cluster of Streptomyces clavuligerus

Francisco J. Pérez-Llarena,1 Antonio Rodríguez-García,1 Francisco J. Enguita,1 Juan F. Martín,1,2 and Paloma Liras1,2,*

Area of Microbiology, Faculty of Biology, University of León, 24071 León,1 and Institute of Biotechnology INBIOTEC, Scientific Park of León, 24006 León,2 Spain

Received 4 May 1998/Accepted 23 June 1998

Three open reading frames (ORFs) have been located downstream of cefE in the cephamycin C gene cluster of Streptomyces clavuligerus. ORF13 (pcd) encodes a 496-amino-acid protein (molecular weight [MW], 52,488) with an N-terminal amino acid sequence identical to that of pure piperideine-6-carboxylate dehydrogenase. ORF14 (cmcT) encodes a 523-amino-acid protein (MW, 54,232) analogous to Streptomyces proteins for efflux and resistance to antibiotics. ORF15 (pbp74) encodes a high molecular weight penicillin-binding protein (MW, 74,094).

* Corresponding author. Mailing address: Area of Microbiology, Faculty of Biology, University of León, 24071 León, Spain. Phone: 34-987-291504. Fax: 34-987-291506. E-mail: degplp{at}unileon.es.

Journal of Bacteriology, September 1998, p. 4753-4756, Vol. 180, No. 17
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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