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 Previous Article

Journal of Bacteriology, September 1998, p. 4760-4763, Vol. 180, No. 17
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Transcriptional Activation of the Bacillus subtilis spoIIG Promoter by the Response Regulator Spo0A Is Independent of the C-Terminal Domain of the RNA Polymerase Alpha Subunit

Dean A. Rowe-Magnus,1 Mario Mencía,2 Fernando Rojo,3 Margarita Salas,2 and George B. Spiegelman1,*

Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada V6T 1Z3,1 and Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM), Universidad Autónoma,2 and Centro Nacional de Biotecnología (CSIC), Campus de la Universidad Autónoma,3 Cantoblanco 28049, Madrid, Spain

Received 1 May 1998/Accepted 6 July 1998

In vitro transcription from the spoIIG promoter by Bacillus subtilis RNA polymerase reconstituted with wild-type alpha subunits and with C-terminal deletion mutants of the alpha subunit was equally stimulated by the response regulator Spo0A. Some differences in the structure of open complexes formed by RNA polymerase containing alpha subunit mutants were noted, although the wild-type and mutant polymerases appeared to use the same initiation mechanism.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of British Columbia, Vancouver V6T 1Z3 Canada. Phone: (604) 822-2036. Fax: (604) 822-6041. E-mail: spie{at}unixg.ubc.ca.

Journal of Bacteriology, September 1998, p. 4760-4763, Vol. 180, No. 17
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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  • Kumar, A., Buckner Starke, C., DeZalia, M., Moran, C. P. Jr. (2004). Surfaces of Spo0A and RNA Polymerase Sigma Factor A That Interact at the spoIIG Promoter in Bacillus subtilis. J. Bacteriol. 186: 200-206 [Abstract] [Full Text]  


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