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Journal of Bacteriology, September 1998, p. 4843-4849, Vol. 180, No. 18
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The ggpS Gene from Synechocystis sp. Strain PCC 6803 Encoding Glucosyl-Glycerol-Phosphate Synthase Is Involved in Osmolyte Synthesis

Kay Marin, Ellen Zuther,dagger Thomas Kerstan, Anja Kunert, and Martin Hagemann*

Universität Rostock, FB Biologie, D-18051 Rostock, Germany

Received 4 May 1998/Accepted 17 July 1998

A salt-sensitive mutant of Synechocystis sp. strain PCC 6803 defective in the synthesis of the compatible solute glucosylglycerol (GG) was used to search for the gene encoding GG-phosphate synthase (GGPS), the key enzyme in GG synthesis. Cloning and sequencing of the mutated region and the corresponding wild-type region revealed that a deletion of about 13 kb occurred in the genome of mutant 11. This deletion affected at least 10 open reading frames, among them regions coding for proteins showing similarities to trehalose (otsA homolog)- and glycerol-3-phosphate-synthesizing enzymes. After construction and characterization of mutants defective in these genes, it became obvious that an otsA homolog (sll1566) (T. Kaneko et al., DNA Res. 3:109-136, 1996) encodes GGPS, since only the mutant affected in sll1566 showed salt sensitivity combined with a complete absence of GG accumulation. Furthermore, the overexpression of sll1566 in Escherichia coli led to the appearance of GGPS activity in the heterologous host. The overexpressed protein did not show the salt dependence that is characteristic for the GGPS in crude protein extracts of Synechocystis.

* Corresponding author. Mailing address: Universität Rostock, FB Biologie, Doberaner Str. 143, D-18051 Rostock, Germany. Phone: 49-381-4942076. Fax: 49-381-4942079. E-mail: mh{at}boserv.bio4.uni-rostock.de.

dagger Present address: University of Chicago, Department of Molecular Genetics and Cell Biology, Chicago, IL 60637.

Journal of Bacteriology, September 1998, p. 4843-4849, Vol. 180, No. 18
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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