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Journal of Bacteriology, September 1998, p. 4850-4855, Vol. 180, No. 18
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Transformation of the Lyme Disease Spirochete Borrelia burgdorferi with Heterologous DNA

Brian Stevenson,* James L. Bono, Abdallah Elias, Kit Tilly, and Patricia Rosa

Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840

Received 6 May 1998/Accepted 22 July 1998

Studies of the spirochete Borrelia burgdorferi have been hindered by the scarcity of genetic tools that can be used in these bacteria. For the first time, a method has been developed by which heterologous DNA (DNA without a naturally occurring B. burgdorferi homolog) can be introduced into and persistently maintained by B. burgdorferi. This technique uses integration of circular DNA into the bacterial genome via a single-crossover event. The ability to transform B. burgdorferi with heterologous DNA will now permit a wide range of experiments on the biology of these bacteria and their involvement in the many facets of Lyme disease.

* Corresponding author. Present address: Department of Microbiology and Immunology, University of Kentucky College of Medicine, MS415 Medical Center, Lexington, KY 40536-0084. Phone: (606) 257-9358. Fax: (606) 257-8994. E-mail: bstev0{at}pop.uky.edu.

Journal of Bacteriology, September 1998, p. 4850-4855, Vol. 180, No. 18
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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