This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stibitz, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stibitz, S.

 Previous Article  |  Next Article 

Journal of Bacteriology, September 1998, p. 4963-4966, Vol. 180, No. 18
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

IS481 and IS1002 of Bordetella pertussis Create a 6-Base-Pair Duplication upon Insertion at a Consensus Target Site

Scott Stibitz*

Division of Bacterial Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892

Received 23 February 1998/Accepted 12 July 1998

The insertion sequence IS481 and its isoform IS1002 have been observed to transpose into the bvgAS locus of Bordetella pertussis, for which the DNA sequence has previously been determined. Upon insertion of IS481 at three different sites and IS1002 at one site, a 6-bp sequence originally present was found at the junction of bvg and insertion sequence DNA. This indicates that, contrary to prior reports, IS481 and IS1002 do create a duplication upon insertion. In this light, examination of these and other examples of IS481 and IS1002 reported in the literature leads to the observation that the 6-bp recognition sequence usually fits the consensus NCTAGN. The near-palindromic nature of this sequence, when directly repeated at the ends of IS481 or IS1002, apparently led to the interpretation that 5 of these base pairs were part of the terminal inverted repeats flanking these elements.

* Mailing address: Division of Bacterial Products, Center for Biologics Evaluation and Research, Food and Drug Administration, 8800 Rockville Pike, Bethesda, MD 20892. Phone: (301) 496-1785. Fax: (301) 402-2776. E-mail: stibitz{at}helix.nih.gov.

Journal of Bacteriology, September 1998, p. 4963-4966, Vol. 180, No. 18
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Dalet, K., Weber, C., Guillemot, L., Njamkepo, E., Guiso, N. (2004). Characterization of Adenylate Cyclase-Hemolysin Gene Duplication in a Bordetella pertussis Isolate. Infect. Immun. 72: 4874-4877 [Abstract] [Full Text]  
  • Holmes, D. S., Zhao, H.-L., Levican, G., Ratouchniak, J., Bonnefoy, V., Varela, P., Jedlicki, E. (2001). ISAfe1, an ISL3 Family Insertion Sequence from Acidithiobacillus ferrooxidans ATCC 19859. J. Bacteriol. 183: 4323-4329 [Abstract] [Full Text]  
  • Reischl, U., Lehn, N., Sanden, G. N., Loeffelholz, M. J. (2001). Real-Time PCR Assay Targeting IS481 of Bordetella pertussis and Molecular Basis for Detecting Bordetella holmesii. J. Clin. Microbiol. 39: 1963-1966 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»