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Journal of Bacteriology, September 1998, p. 4982-4986, Vol. 180, No. 18
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Heterologous Expression of the Desulfovibrio gigas [NiFe] Hydrogenase in Desulfovibrio fructosovorans MR400

Marc Rousset, Valérie Magro, Nicole Forget, Bruno Guigliarelli, Jean-Pierre Belaich, and E. Claude Hatchikian*

Unité de Bioénergétique et Ingénierie des Protéines, IBSM, CNRS, 13402 Marseille Cedex 20, France

Received 9 February 1998/Accepted 7 July 1998

The ability of Desulfovibrio fructosovorans MR400 Delta hynABC to express the heterologous cloned [NiFe] hydrogenase of Desulfovibrio gigas was investigated. The [NiFe] hydrogenase operon from D. gigas, hynABCD, was cloned, sequenced, and introduced into D. fructosovorans MR400. A portion of the recombinant heterologous [NiFe] hydrogenase was totally matured, exhibiting catalytic and spectroscopic properties identical to those of the native D. gigas protein. A chimeric operon containing hynAB from D. gigas and hynC from D. fructosovorans placed under the control of the D. fructosovorans hynAp promoter was constructed and expressed in D. fructosovorans MR400. Under these conditions, the same level of activity was obtained as with the D. gigas hydrogenase operon.

* Corresponding author. Mailing address: Unité de Bioénergétique et Ingéniérie des Protéines, IBSM, CNRS, 31, Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France. Phone: 04 91 16 41 45. Fax: 04 91 16 45 78. E-mail: hatch{at}ibsm.cnrs-mrs.fr.

Journal of Bacteriology, September 1998, p. 4982-4986, Vol. 180, No. 18
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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