This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gouffi, K.
Right arrow Articles by Blanco, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gouffi, K.
Right arrow Articles by Blanco, C.

 Previous Article  |  Next Article 

Journal of Bacteriology, October 1998, p. 5044-5051, Vol. 180, No. 19
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Sucrose Is a Nonaccumulated Osmoprotectant in Sinorhizobium meliloti

Kamila Gouffi,1 Vianney Pichereau,1 Jean-Paul Rolland,2 Daniel Thomas,2 Théophile Bernard,1 and Carlos Blanco1,*

Groupe Membranes et Osmorégulation1 and Groupe Canaux et Récepteurs Membranaires,2 UPRES-A CNRS 6026, Université de Rennes 1, Campus de Beaulieu, F35042, Rennes, France

Received 8 May 1998/Accepted 22 July 1998

Intracellular accumulation of sucrose in response to lowered water activity seems to occur only in photosynthetic organisms. Here we demonstrate, for the first time, the potent ability of this common sugar, supplied exogenously, to reduce growth inhibition of Sinorhizobium meliloti cells in media of inhibitory osmolarity. Independently of the nature of the growth substrates and the osmotic agent, sucrose appears particularly efficient in promoting the recovery of cytoplasmic volume after plasmolysis. Surprisingly, sucrose is not accumulated by the bacteria at an osmotically efficient level. Instead, it strongly stimulates the accumulation of the main endogenous osmolytes glutamate and N-acetylglutaminylglutamine amide (NAGGN). Examining cell volume changes during the hyperosmotic treatment, we found a close correlation between the enhancement of the osmotically active solute pool and the increase in cell volume. Sucrose shares several features with ectoine, another nonaccumulated osmoprotectant for S. meliloti. Overall, osmoregulation in S. meliloti appears to be strongly divergent from that in most bacteria.

* Corresponding author. Mailing address: Groupe Membranes et Osmorégulation, UPRES-A CNRS 6026, Université de Rennes 1, Campus de Beaulieu, Av. du Général Leclerc, F35042 Rennes, France. Phone and fax: 33 (0)2 99 28 61 40. E-mail: Carlos.Blanco{at}univ-rennes1.fr.

Journal of Bacteriology, October 1998, p. 5044-5051, Vol. 180, No. 19
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Vriezen, J. A. C., de Bruijn, F. J., Nusslein, K. (2007). Responses of Rhizobia to Desiccation in Relation to Osmotic Stress, Oxygen, and Temperature. Appl. Environ. Microbiol. 73: 3451-3459 [Full Text]  
  • Barra, L., Fontenelle, C., Ermel, G., Trautwetter, A., Walker, G. C., Blanco, C. (2006). Interrelations between Glycine Betaine Catabolism and Methionine Biosynthesis in Sinorhizobium meliloti Strain 102F34.. J. Bacteriol. 188: 7195-7204 [Abstract] [Full Text]  
  • Jebbar, M., Sohn-Bosser, L., Bremer, E., Bernard, T., Blanco, C. (2005). Ectoine-Induced Proteins in Sinorhizobium meliloti Include an Ectoine ABC-Type Transporter Involved in Osmoprotection and Ectoine Catabolism. J. Bacteriol. 187: 1293-1304 [Abstract] [Full Text]  
  • Goude, R., Renaud, S., Bonnassie, S., Bernard, T., Blanco, C. (2004). Glutamine, Glutamate, and {alpha}-Glucosylglycerate Are the Major Osmotic Solutes Accumulated by Erwinia chrysanthemi Strain 3937. Appl. Environ. Microbiol. 70: 6535-6541 [Abstract] [Full Text]  
  • Gouffi, K., Bernard, T., Blanco, C. (2000). Osmoprotection by Pipecolic Acid in Sinorhizobium meliloti: Specific Effects of D and L Isomers. Appl. Environ. Microbiol. 66: 2358-2364 [Abstract] [Full Text]  
  • Gouffi, K., Pica, N., Pichereau, V., Blanco, C. (1999). Disaccharides as a New Class of Nonaccumulated Osmoprotectants for Sinorhizobium meliloti. Appl. Environ. Microbiol. 65: 1491-1500 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»