This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chang, B.
Right arrow Articles by Yoshida, S.-i.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chang, B.
Right arrow Articles by Yoshida, S.-i.

 Previous Article  |  Next Article 

Journal of Bacteriology, October 1998, p. 5094-5101, Vol. 180, No. 19
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Filamentous Bacteriophages of Vibrio parahaemolyticus as a Possible Clue to Genetic Transmission

Bin Chang, Hatsumi Taniguchi,* Hiroshi Miyamoto, and Shin-ichi Yoshida

Department of Microbiology, School of Medicine, University of Occupational and Environmental Health, Iseigaoka 1-1, Yahatanishiku, Kitakyushu 807-8555, Japan

Received 27 April 1998/Accepted 3 August 1998

We have previously reported the isolation and characterization of two filamentous bacteriophages of Vibrio parahaemolyticus, designated Vf12 and Vf33. In this study, to understand the potential of these phages as tools for genetic transmission, we investigated the gene structures of replicative-form (RF) DNAs of their genomes and the distribution of these DNAs on chromosomal and extrachromosomal DNAs. The 7,965-bp nucleotide sequences of Vf12 and Vf33 were determined. An analysis of the overall gene structures revealed that Vf12 and Vf33 had conserved regions and distinctive regions. The gene organization of their conserved regions was similar to that of CTX phage of Vibrio cholerae and coliphage Ff of Escherichia coli, while their distinctive regions were characteristic of Vf12 and Vf33 phage genomes. Southern blot hybridization testing revealed that the filamentous phage genomes integrated into chromosomal DNA of V. parahaemolyticus at the distinctive region of the phage genome and were also distributed on some plasmids of V. parahaemolyticus and total cellular DNAs of one Vibrio damsela and one nonagglutinable Vibrio strain tested. These results strongly suggest the possibilities of genetic interaction among the bacteriophage Vf12 and Vf33 genomes and chromosomal and plasmid-borne DNAs of V. parahaemolyticus strains and of genetic transmission among strains through these filamentous phages.

* Corresponding author. Mailing address: Department of Microbiology, School of Medicine, University of Occupational and Environmental Health, Iseigaoka, Yahatanishiku, Kitakyushu 807-8555, Japan. Phone: 81-93-691-7242. Fax: 81-93-602-4799. E-mail: hatsumi{at}med.uoeh-u.ac.jp.

Journal of Bacteriology, October 1998, p. 5094-5101, Vol. 180, No. 19
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Hazen, T. H., Wu, D., Eisen, J. A., Sobecky, P. A. (2007). Sequence Characterization and Comparative Analysis of Three Plasmids Isolated from Environmental Vibrio spp.. Appl. Environ. Microbiol. 73: 7703-7710 [Abstract] [Full Text]  
  • Kawasaki, T., Nagata, S., Fujiwara, A., Satsuma, H., Fujie, M., Usami, S., Yamada, T. (2007). Genomic Characterization of the Filamentous Integrative Bacteriophages {phi}RSS1 and {phi}RSM1, Which Infect Ralstonia solanacearum. J. Bacteriol. 189: 5792-5802 [Abstract] [Full Text]  
  • Harth, E., Romero, J., Torres, R., Espejo, R. T. (2007). Intragenomic heterogeneity and intergenomic recombination among Vibrio parahaemolyticus 16S rRNA genes. Microbiology 153: 2640-2647 [Abstract] [Full Text]  
  • Thompson, F. L., Iida, T., Swings, J. (2004). Biodiversity of Vibrios. Microbiol. Mol. Biol. Rev. 68: 403-431 [Abstract] [Full Text]  
  • Campos, J., Martinez, E., Suzarte, E., Rodriguez, B. L., Marrero, K., Silva, Y., Ledon, T., del Sol, R., Fando, R. (2003). VGJ{phi}, a Novel Filamentous Phage of Vibrio cholerae, Integrates into the Same Chromosomal Site as CTX{phi}. J. Bacteriol. 185: 5685-5696 [Abstract] [Full Text]  
  • Boyd, E. F., Heilpern, A. J., Waldor, M. K. (2000). Molecular Analyses of a Putative CTXphi Precursor and Evidence for Independent Acquisition of Distinct CTXphi s by Toxigenic Vibrio cholerae. J. Bacteriol. 182: 5530-5538 [Abstract] [Full Text]  
  • Nasu, H., Iida, T., Sugahara, T., Yamaichi, Y., Park, K.-S., Yokoyama, K., Makino, K., Shinagawa, H., Honda, T. (2000). A Filamentous Phage Associated with Recent Pandemic Vibrio parahaemolyticus O3:K6 Strains. J. Clin. Microbiol. 38: 2156-2161 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»