This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Oliver, D.
Right arrow Articles by Sarker, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Oliver, D.
Right arrow Articles by Sarker, S.

 Previous Article  |  Next Article 

Journal of Bacteriology, October 1998, p. 5240-5242, Vol. 180, No. 19
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Regulation of Escherichia coli secA by Cellular Protein Secretion Proficiency Requires an Intact Gene X Signal Sequence and an Active Translocon

Donald Oliver,* Jessica Norman,dagger and Shameema Sarker

Department of Molecular Biology and Biochemistry, Wesleyan University, Middletown, Connecticut 06459

Received 27 May 1998/Accepted 3 August 1998

secA is translationally regulated by the protein secretion proficiency state of the Escherichia coli cell. This regulation was explored by making signal sequence mutations in the gene upstream of secA, gene X, which promotes secA translational coupling. Gene X signal sequence mutants were constitutive for secA expression, while prlA alleles partially restored secA regulation. These results show that interaction of the pre-gene X protein with the translocon is required for proper secA regulation. Furthermore, gene X signal sequence mutations disrupted secA regulation only in the cis configuration. We propose that nascent pre-gene X protein interacts with the translocon during its secretion to constitute the secretion sensor.

* Corresponding author. Mailing address: Department of Molecular Biology and Biochemistry, Wesleyan University, Middletown, CT 06459. Phone: (860) 685-3556. Fax: (860) 685-2141. E-mail: doliver{at}wesleyan.edu.

dagger Present address: Laboratory of Pathology, National Cancer Institute, Bethesda, MD 20892.

Journal of Bacteriology, October 1998, p. 5240-5242, Vol. 180, No. 19
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Beringer, M. (2008). Modulating the activity of the peptidyl transferase center of the ribosome. RNA 14: 795-801 [Abstract] [Full Text]  
  • Sugai, R., Takemae, K., Tokuda, H., Nishiyama, K.-i. (2007). Topology Inversion of SecG Is Essential for Cytosolic SecA-dependent Stimulation of Protein Translocation. J. Biol. Chem. 282: 29540-29548 [Abstract] [Full Text]  
  • Nakatogawa, H., Murakami, A., Mori, H., Ito, K. (2005). SecM facilitates translocase function of SecA by localizing its biosynthesis. Genes Dev. 19: 436-444 [Abstract] [Full Text]  
  • Collier, J., Bohn, C., Bouloc, P. (2004). SsrA Tagging of Escherichia coli SecM at Its Translation Arrest Sequence. J. Biol. Chem. 279: 54193-54201 [Abstract] [Full Text]  
  • Murakami, A., Nakatogawa, H., Ito, K. (2004). Translation arrest of SecM is essential for the basal and regulated expression of SecA. Proc. Natl. Acad. Sci. USA 101: 12330-12335 [Abstract] [Full Text]  
  • Sunohara, T., Jojima, K., Tagami, H., Inada, T., Aiba, H. (2004). Ribosome Stalling during Translation Elongation Induces Cleavage of mRNA Being Translated in Escherichia coli. J. Biol. Chem. 279: 15368-15375 [Abstract] [Full Text]  
  • Butkus, M. E., Prundeanu, L. B., Oliver, D. B. (2003). Translocon """Pulling""" of Nascent SecM Controls the Duration of Its Translational Pause and Secretion-Responsive secA Regulation. J. Bacteriol. 185: 6719-6722 [Abstract] [Full Text]  
  • WILLIAMS, E. H., FOX, T. D. (2003). Antagonistic signals within the COX2 mRNA coding sequence control its translation in Saccharomyces cerevisiae mitochondria. RNA 9: 419-431 [Abstract] [Full Text]  
  • Sarker, S., Oliver, D. (2002). Critical Regions of secM That Control Its Translation and Secretion and Promote Secretion-Specific secA Regulation. J. Bacteriol. 184: 2360-2369 [Abstract] [Full Text]  
  • Sarker, S., Rudd, K. E., Oliver, D. (2000). Revised Translation Start Site for secM Defines an Atypical Signal Peptide That Regulates Escherichia coli secA Expression. J. Bacteriol. 182: 5592-5595 [Abstract] [Full Text]  
  • Schmidt, M. O., Brosh, R. M. Jr., Oliver, D. B. (2001). Escherichia coli SecA Helicase Activity Is Not Required in Vivo for Efficient Protein Translocation or Autogenous Regulation. J. Biol. Chem. 276: 37076-37085 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»