This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Borghese, R. Articles by Melandri, B. A. Search for Related Content PubMed PubMed Citation Articles by Borghese, R. Articles by Melandri, B. A.

 Previous Article  |  Next Article 

J. Bacteriol., 01 1998, 416-421, Vol 180, No. 2
Copyright © 1998, American Society for Microbiology

The ATP synthase atpHAGDC (F1) operon from Rhodobacter capsulatus [In Process Citation]

R Borghese, M Crimi, L Fava and BA Melandri
Department of Biology, University of Bologna, Italy.

The atpHAGDC operon of Rhodobacter capsulatus, containing the five genes coding for the F1 sector of the ATP synthase, has been cloned and sequenced. The promoter region has been defined by primer extension analysis. It was not possible to obtain viable cells carrying atp deletions in the R. capsulatus chromosome, indicating that genes coding for ATP synthase are essential, at least under the growth conditions tested. We were able to circumvent this problem by combining gene transfer agent transduction with conjugation. This method represents an easy way to construct strains carrying mutations in indispensable genes.

This article has been cited by other articles:

• Baker, S. C., Ferguson, S. J., Ludwig, B., Page, M. D., Richter, O.-M. H., van Spanning, R. J. M. (1998). Molecular Genetics of the Genus Paracoccus: Metabolically Versatile Bacteria with Bioenergetic Flexibility. Microbiol. Mol. Biol. Rev. 62: 1046-1078 [Abstract] [Full Text]