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J. Bacteriol., 01 1998, 426-429, Vol 180, No. 2
Copyright © 1998, American Society for Microbiology

Construction and characterization of a Helicobacter pylori clpB mutant and role of the gene in the stress response [In Process Citation]

E Allan, P Mullany and S Tabaqchali
Department of Medical Microbiology, St. Bartholomew's and the Royal London School of Medicine and Dentistry, West Smithfield, United Kingdom. e.allan@mds.qmw.ac.uk

Antiserum raised against whole Helicobacter pylori cells identified a novel 94-kDa antigen. The nucleotide sequence of the gene encoding the 94-kDa antigen was determined, and analysis of the deduced amino acid sequence revealed structural features typical of the ClpB ATPase family of stress response proteins. An isogenic H. pylori clpB mutant showed increased sensitivity to high-temperature stress, indicating that the clpB gene product functions as a stress response protein in H. pylori.


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