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Journal of Bacteriology, October 1998, p. 5443-5447, Vol. 180, No. 20
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Influence of the MexAB-OprM Multidrug Efflux System on Quorum Sensing in Pseudomonas aeruginosa

Kelly Evans,1 Luciano Passador,2 Ramakrishnan Srikumar,1 Eric Tsang,1 Jonathon Nezezon,2 and Keith Poole1,*

Department of Microbiology and Immunology, Queen's University, Kingston, Ontario K7L 3N6, Canada,1 and Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, New York 146422

Received 10 June 1998/Accepted 11 August 1998

Pseudomonas aeruginosa nalB mutants which hyperexpress the MexAB-OprM multidrug efflux system produce reduced levels of several extracellular virulence factors known to be regulated by quorum sensing. Such mutants also produce less acylated homoserine lactone autoinducer PAI-1, consistent with an observed reduction in lasI expression. These data suggest that PAI-1 is a substrate for MexAB-OprM, and its resulting exclusion from cells hyperexpressing MexAB-OprM limits PAI-1-dependent activation of lasI and the virulence genes.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Queen's University, Kingston, Ontario K7L 3N6, Canada. Phone: 613-545-6677. Fax: 613-545-6796. E-mail: poolek{at}post.queensu.ca.

Journal of Bacteriology, October 1998, p. 5443-5447, Vol. 180, No. 20
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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