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 Previous Article

Journal of Bacteriology, October 1998, p. 5489-5494, Vol. 180, No. 20
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The Nine Genes of the Nocardia lactamdurans Cephamycin Cluster Are Transcribed into Large mRNAs from Three Promoters, Two of Them Located in a Bidirectional Promoter Region

Francisco J. Enguita,1 Juan Jose R. Coque,1 Paloma Liras,1,2 and Juan F. Martin1,2,*

Area of Microbiology, Faculty of Biology, University of León, 24071 León,1 and Institute of Biotechnology INBIOTEC, Parque Científico de León, 24006 León,2 Spain

Received 12 May 1998/Accepted 29 July 1998

The nine biosynthesis genes of the Nocardia lactamdurans cephamycin cluster are expressed as three different mRNAs initiating at promoters latp, cefDp, and pcbABp, as shown by low-resolution S1 nuclease protection assays and Northern blotting analysis. Bidirectional expression occurred from divergent promoters (latp and cefDp) located in a 629-bp intergenic region that contains three heptameric direct repeats similar to those recognized by members of the SARP (Streptomyces antibiotic regulatory proteins) family. The lat gene is transcribed in a single monocistronic transcript initiating at latp. A second unusually long polycistronic mRNA (more than 16 kb) corresponding to six biosynthesis genes (pcbAB, pcbC, cmcI, cmcJ, cefF, and cmcH) started at pcbABp. A third polycistronic mRNA corresponding to the cefD and cefE genes started at cefDp.

* Corresponding author. Mailing address: Area of Microbiology, Faculty of Biology, University of León, 24071 León, Spain. Phone: (34 987) 291505. Fax: (34 987) 291506. E-mail: degjmm{at}unileon.es.

Journal of Bacteriology, October 1998, p. 5489-5494, Vol. 180, No. 20
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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