This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lazar, S. W.
Right arrow Articles by Kolter, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lazar, S. W.
Right arrow Articles by Kolter, R.

 Previous Article  |  Next Article 

Journal of Bacteriology, November 1998, p. 5704-5711, Vol. 180, No. 21
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Role of the Escherichia coli SurA Protein in Stationary-Phase Survival

Sara W. Lazar,dagger Marta Almirón,Dagger Antonio Tormo,§ and Roberto Kolter*

Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115

Received 10 July 1998/Accepted 28 August 1998

SurA is a periplasmic peptidyl-prolyl isomerase required for the efficient folding of extracytoplasmic proteins. Although the surA gene had been identified in a screen for mutants that failed to survive in stationary phase, the role played by SurA in stationary-phase survival remained unknown. The results presented here demonstrate that the survival defect of surA mutants is due to their inability to grow at elevated pH in the absence of sigma S. When cultures of Escherichia coli were grown in peptide-rich Luria-Bertani medium, the majority of the cells lost viability during the first two to three days of incubation in stationary phase as the pH rose to pH 9. At this time the surviving cells resumed growth. In cultures of surA rpoS double mutants the survivors lysed as they attempted to resume growth at the elevated pH. Cells lacking penicillin binding protein 3 and sigma S had a survival defect similar to that of surA rpoS double mutants, suggesting that SurA foldase activity is important for the proper assembly of the cell wall-synthesizing apparatus.

* Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Ave., Boston, MA 02115. Phone: (617) 432-1776. Fax: (617) 738-7664. E-mail: kolter{at}mbcrr.harvard.edu.

dagger Present address: MGH-NMR Center, Massachusettes General Hospital, Charlestown, MA 02129.

Dagger Present address: Instituto de Investigaciones Biotecnologicas, Universidad de San Martin, 1650 San Martin, Argentina.

§ Present address: Departamento de Bioquimica y Biologia Molecular I, Universidad Complutense de Madrid, Madrid, Spain.

Journal of Bacteriology, November 1998, p. 5704-5711, Vol. 180, No. 21
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Nicholson, T. F., Watts, K. M., Hunstad, D. A. (2009). OmpA of Uropathogenic Escherichia coli Promotes Postinvasion Pathogenesis of Cystitis. Infect. Immun. 77: 5245-5251 [Abstract] [Full Text]  
  • Castanie-Cornet, M.-P., Cam, K., Jacq, A. (2006). RcsF Is an Outer Membrane Lipoprotein Involved in the RcsCDB Phosphorelay Signaling Pathway in Escherichia coli.. J. Bacteriol. 188: 4264-4270 [Abstract] [Full Text]  
  • Hicks, W. M., Kotlajich, M. V., Visick, J. E. (2005). Recovery from long-term stationary phase and stress survival in Escherichia coli require the L-isoaspartyl protein carboxyl methyltransferase at alkaline pH. Microbiology 151: 2151-2158 [Abstract] [Full Text]  
  • Cuny, C., Dukan, L., Fraysse, L., Ballesteros, M., Dukan, S. (2005). Investigation of the First Events Leading to Loss of Culturability during Escherichia coli Starvation: Future Nonculturable Bacteria Form a Subpopulation. J. Bacteriol. 187: 2244-2248 [Abstract] [Full Text]  
  • Stokes, N. R., Murray, H. D., Subramaniam, C., Gourse, R. L., Louis, P., Bartlett, W., Miller, S., Booth, I. R. (2003). A role for mechanosensitive channels in survival of stationary phase: Regulation of channel expression by RpoS. Proc. Natl. Acad. Sci. USA 100: 15959-15964 [Abstract] [Full Text]  
  • Goffin, C., Ghuysen, J.-M. (2002). Biochemistry and Comparative Genomics of SxxK Superfamily Acyltransferases Offer a Clue to the Mycobacterial Paradox: Presence of Penicillin-Susceptible Target Proteins versus Lack of Efficiency of Penicillin as Therapeutic Agent. Microbiol. Mol. Biol. Rev. 66: 702-738 [Abstract] [Full Text]  
  • Stancik, L. M., Stancik, D. M., Schmidt, B., Barnhart, D. M., Yoncheva, Y. N., Slonczewski, J. L. (2002). pH-Dependent Expression of Periplasmic Proteins and Amino Acid Catabolism in Escherichia coli. J. Bacteriol. 184: 4246-4258 [Abstract] [Full Text]  
  • Pease, A. J., Roa, B. R., Luo, W., Winkler, M. E. (2002). Positive Growth Rate-Dependent Regulation of the pdxA, ksgA, and pdxB Genes of Escherichia coli K-12. J. Bacteriol. 184: 1359-1369 [Abstract] [Full Text]  
  • Rizzitello, A. E., Harper, J. R., Silhavy, T. J. (2001). Genetic Evidence for Parallel Pathways of Chaperone Activity in the Periplasm of Escherichia coli. J. Bacteriol. 183: 6794-6800 [Abstract] [Full Text]  
  • Webb, H. M., Ruddock, L. W., Marchant, R. J., Jonas, K., Klappa, P. (2001). Interaction of the Periplasmic Peptidylprolyl cis-trans Isomerase SurA with Model Peptides. THE N-TERMINAL REGION OF SurA IS ESSENTIAL AND SUFFICIENT FOR PEPTIDE BINDING. J. Biol. Chem. 276: 45622-45627 [Abstract] [Full Text]  
  • Keer, J., Smeulders, M. J., Gray, K. M., Williams, H. D. (2000). Mutants of Mycobacterium smegmatis impaired in stationary-phase survival. Microbiology 146: 2209-2217 [Abstract] [Full Text]  
  • Sydenham, M., Douce, G., Bowe, F., Ahmed, S., Chatfield, S., Dougan, G. (2000). Salmonella enterica Serovar Typhimurium surA Mutants Are Attenuated and Effective Live Oral Vaccines. Infect. Immun. 68: 1109-1115 [Abstract] [Full Text]  
  • Blankenhorn, D., Phillips, J., Slonczewski, J. L. (1999). Acid- and Base-Induced Proteins during Aerobic and Anaerobic Growth of Escherichia coli Revealed by Two-Dimensional Gel Electrophoresis. J. Bacteriol. 181: 2209-2216 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»