This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An author's correction has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Padilla, P. A.
Right arrow Articles by Werner-Washburne, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Padilla, P. A.
Right arrow Articles by Werner-Washburne, M.

 Previous Article  |  Next Article 

Journal of Bacteriology, November 1998, p. 5718-5726, Vol. 180, No. 21
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The Highly Conserved, Coregulated SNO and SNZ Gene Families in Saccharomyces cerevisiae Respond to Nutrient Limitation

Pamela A. Padilla, Edwina K. Fuge, Matthew E. Crawford, Allison Errett, and Margaret Werner-Washburne*

Department of Biology, University of New Mexico, Albuquerque, New Mexico 87131

Received 20 July 1998/Accepted 31 August 1998

SNZ1, a member of a highly conserved gene family, was first identified through studies of proteins synthesized in stationary-phase yeast cells. There are three SNZ genes in Saccharomyces cerevisiae, each of which has another highly conserved gene, named SNO (SNZ proximal open reading frame), upstream. The DNA sequences and relative positions of SNZ and SNO genes have been phylogenetically conserved. This report details studies of the expression of the SNZ-SNO gene pairs under various conditions and phenotypic analysis of snz-sno mutants. An analysis of total RNA was used to determine that adjacent SNZ-SNO gene pairs are coregulated. SNZ2/3 and SNO2/3 mRNAs are induced prior to the diauxic shift and decrease in abundance during the postdiauxic phase, when SNZ1 and SNO1 are induced. In snz2 snz3 mutants, SNZ1 mRNA is induced prior to the diauxic shift, when SNZ2/3 mRNAs are normally induced. Under nitrogen-limiting conditions, SNZ1 mRNAs accumulate in tryptophan, adenine, and uracil auxotrophs but not in prototrophic strains, indicating that induction occurs in response to the limitation of specific nutrients. Strains carrying deletions in all SNZ-SNO gene pairs are viable, but snz1 and sno1 mutants are sensitive to 6-azauracil (6-AU), an inhibitor of purine and pyrimidine biosynthetic enzymes, and methylene blue, a producer of singlet oxygen. The conservation of sequence and chromosomal position, the coregulation and pattern of expression of SNZ1 and SNO1 genes, and the sensitivity of snz1 and sno1 mutants to 6-AU support the hypothesis that the associated proteins are part of an ancient response to nutrient limitation.

* Corresponding author. Mailing address: Department of Biology, University of New Mexico, Albuquerque, NM 87131. Phone: (505) 277-9338. Fax: (505) 277-0304. E-mail: maggieww{at}unm.edu.

Journal of Bacteriology, November 1998, p. 5718-5726, Vol. 180, No. 21
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Craig, J. P., Bekal, S., Hudson, M., Domier, L., Niblack, T., Lambert, K. N. (2008). Analysis of a Horizontally Transferred Pathway Involved in Vitamin B6 Biosynthesis from the Soybean Cyst Nematode Heterodera glycines. Mol Biol Evol 25: 2085-2098 [Abstract] [Full Text]  
  • Nishizawa, M., Komai, T., Morohashi, N., Shimizu, M., Toh-e, A. (2008). Transcriptional Repression by the Pho4 Transcription Factor Controls the Timing of SNZ1 Expression. Eukaryot Cell 7: 949-957 [Abstract] [Full Text]  
  • Wagner, S., Bernhardt, A., Leuendorf, J. E., Drewke, C., Lytovchenko, A., Mujahed, N., Gurgui, C., Frommer, W. B., Leistner, E., Fernie, A. R., Hellmann, H. (2006). Analysis of the Arabidopsis rsr4-1/pdx1-3 Mutant Reveals the Critical Function of the PDX1 Protein Family in Metabolism, Development, and Vitamin B6 Biosynthesis. Plant Cell 18: 1722-1735 [Abstract] [Full Text]  
  • Gengenbacher, M., Fitzpatrick, T. B., Raschle, T., Flicker, K., Sinning, I., Muller, S., Macheroux, P., Tews, I., Kappes, B. (2006). Vitamin B6 Biosynthesis by the Malaria Parasite Plasmodium falciparum: BIOCHEMICAL AND STRUCTURAL INSIGHTS. J. Biol. Chem. 281: 3633-3641 [Abstract] [Full Text]  
  • Zhu, J., Burgner, J. W., Harms, E., Belitsky, B. R., Smith, J. L. (2005). A New Arrangement of ({beta}/{alpha})8 Barrels in the Synthase Subunit of PLP Synthase. J. Biol. Chem. 280: 27914-27923 [Abstract] [Full Text]  
  • Wrenger, C., Eschbach, M.-L., Muller, I. B., Warnecke, D., Walter, R. D. (2005). Analysis of the Vitamin B6 Biosynthesis Pathway in the Human Malaria Parasite Plasmodium falciparum. J. Biol. Chem. 280: 5242-5248 [Abstract] [Full Text]  
  • Stolz, J., Wohrmann, H. J. P., Vogl, C. (2005). Amiloride Uptake and Toxicity in Fission Yeast Are Caused by the Pyridoxine Transporter Encoded by bsu1+ (car1+). Eukaryot Cell 4: 319-326 [Abstract] [Full Text]  
  • Andalis, A. A., Storchova, Z., Styles, C., Galitski, T., Pellman, D., Fink, G. R. (2004). Defects Arising From Whole-Genome Duplications in Saccharomyces cerevisiae. Genetics 167: 1109-1121 [Abstract] [Full Text]  
  • Gray, J. V., Petsko, G. A., Johnston, G. C., Ringe, D., Singer, R. A., Werner-Washburne, M. (2004). "Sleeping Beauty": Quiescence in Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 68: 187-206 [Abstract] [Full Text]  
  • Belli, G., Molina, M. M., Garcia-Martinez, J., Perez-Ortin, J. E., Herrero, E. (2004). Saccharomyces cerevisiae Glutaredoxin 5-deficient Cells Subjected to Continuous Oxidizing Conditions Are Affected in the Expression of Specific Sets of Genes. J. Biol. Chem. 279: 12386-12395 [Abstract] [Full Text]  
  • Gelling, C. L., Piper, M. D. W., Hong, S.-P., Kornfeld, G. D., Dawes, I. W. (2004). Identification of a Novel One-carbon Metabolism Regulon in Saccharomyces cerevisiae. J. Biol. Chem. 279: 7072-7081 [Abstract] [Full Text]  
  • Belitsky, B. R. (2004). Physical and Enzymological Interaction of Bacillus subtilis Proteins Required for De Novo Pyridoxal 5'-Phosphate Biosynthesis. J. Bacteriol. 186: 1191-1196 [Abstract] [Full Text]  
  • Bauer, J. A., Bennett, E. M., Begley, T. P., Ealick, S. E. (2004). Three-dimensional Structure of YaaE from Bacillus subtilis, a Glutaminase Implicated in Pyridoxal-5'-phosphate Biosynthesis. J. Biol. Chem. 279: 2704-2711 [Abstract] [Full Text]  
  • Howard, S. C., Hester, A., Herman, P. K. (2003). The Ras/PKA Signaling Pathway May Control RNA Polymerase II Elongation via the Spt4p/Spt5p Complex in Saccharomyces cerevisiae. Genetics 165: 1059-1070 [Abstract] [Full Text]  
  • Wightman, R., Meacock, P. A. (2003). The THI5 gene family of Saccharomyces cerevisiae: distribution of homologues among the hemiascomycetes and functional redundancy in the aerobic biosynthesis of thiamin from pyridoxine. Microbiology 149: 1447-1460 [Abstract] [Full Text]  
  • Stolz, J., Vielreicher, M. (2003). Tpn1p, the Plasma Membrane Vitamin B6 Transporter of Saccharomyces cerevisiae. J. Biol. Chem. 278: 18990-18996 [Abstract] [Full Text]  
  • Paustian, M. L., May, B. J., Kapur, V. (2002). Transcriptional Response of Pasteurella multocida to Nutrient Limitation. J. Bacteriol. 184: 3734-3739 [Abstract] [Full Text]  
  • Howard, S. C., Chang, Y.-W., Budovskaya, Y. V., Herman, P. K. (2001). The Ras/PKA Signaling Pathway of Saccharomyces cerevisiae Exhibits a Functional Interaction With the Sin4p Complex of the RNA Polymerase II Holoenzyme. Genetics 159: 77-89 [Abstract] [Full Text]  
  • Natarajan, K., Meyer, M. R., Jackson, B. M., Slade, D., Roberts, C., Hinnebusch, A. G., Marton, M. J. (2001). Transcriptional Profiling Shows that Gcn4p Is a Master Regulator of Gene Expression during Amino Acid Starvation in Yeast. Mol. Cell. Biol. 21: 4347-4368 [Abstract] [Full Text]  
  • Ehrenshaft, M., Daub, M. E. (2001). Isolation of PDX2, a Second Novel Gene in the Pyridoxine Biosynthesis Pathway of Eukaryotes, Archaebacteria, and a Subset of Eubacteria. J. Bacteriol. 183: 3383-3390 [Abstract] [Full Text]  
  • Bean, L. E., Dvorachek, W. H. , Jr., Braun, E. L., Errett, A., Saenz, G. S., Giles, M. D., Werner-Washburne, M., Nelson, M. A., Natvig, D. O. (2001). Analysis of the pdx-1 (snz-1/sno-1) Region of the Neurospora crassa Genome: Correlation of Pyridoxine-Requiring Phenotypes With Mutations in Two Structural Genes. Genetics 157: 1067-1075 [Abstract] [Full Text]  
  • Chang, Y.-W., Howard, S. C., Budovskaya, Y. V., Rine, J., Herman, P. K. (2001). The rye Mutants Identify a Role for Ssn/Srb Proteins of the RNA Polymerase II Holoenzyme During Stationary Phase Entry in Saccharomyces cerevisiae. Genetics 157: 17-26 [Abstract] [Full Text]  
  • Shaw, R. J., Reines, D. (2000). Saccharomyces cerevisiae Transcription Elongation Mutants Are Defective in PUR5 Induction in Response to Nucleotide Depletion. Mol. Cell. Biol. 20: 7427-7437 [Abstract] [Full Text]  
  • JIA, M. H., LAROSSA, R. A., LEE, J.-M., RAFALSKI, A., DEROSE, E., GONYE, G., XUE, Z. (2000). Global expression profiling of yeast treated with an inhibitor of amino acid biosynthesis, sulfometuron methyl. Physiol. Genomics 3: 83-92 [Abstract] [Full Text]  
  • Osmani, A. H., May, G. S., Osmani, S. A. (1999). The Extremely Conserved pyroA Gene of Aspergillus nidulans Is Required for Pyridoxine Synthesis and Is Required Indirectly for Resistance to Photosensitizers. J. Biol. Chem. 274: 23565-23569 [Abstract] [Full Text]  
  • Ehrenshaft, M., Bilski, P., Li, M. Y., Chignell, C. F., Daub, M. E. (1999). A highly conserved sequence is a novel gene involved in de novo vitamin B6 biosynthesis. Proc. Natl. Acad. Sci. USA 96: 9374-9378 [Abstract] [Full Text]  
  • Wind-Rotolo, M., Reines, D. (2001). Analysis of Gene Induction and Arrest Site Transcription in Yeast with Mutations in the Transcription Elongation Machinery. J. Biol. Chem. 276: 11531-11538 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»