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Journal of Bacteriology, November 1998, p. 6039-6042, Vol. 180, No. 22
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The Carboxyl Terminus of Protein TraD Adds Specificity and Efficiency to F-Plasmid Conjugative Transfer

J. Ignacio Sastre, Elena Cabezón,dagger and Fernando de la Cruz*

Department of Molecular Biology, University of Cantabria, 39011 Santander, Spain

Received 20 May 1998/Accepted 5 September 1998

We isolated and characterized traD mutants with an altered specificity of interaction with relaxosomes of various conjugative (F and R388) and mobilizable (RSF1010 and ColE1) plasmids. The change in specificity was due to a loss of some amino acids in the carboxyl terminus of TraD that resulted in a broadening of the range of mobilizable relaxosomes at the expense of a decrease in the efficiency of F-plasmid transfer.

* Corresponding author. Mailing address: Department of Molecular Biology, University of Cantabria, Cardenal Herrera Oria s/n, 39011 Santander, Spain. Phone: 34-42-201942. Fax: 34-42-201945. E-mail:delacruz{at}medi.unican.es.

dagger Present address: Laboratory of Molecular Biology of the Medical Research Council, Cambridge, United Kingdom.

Journal of Bacteriology, November 1998, p. 6039-6042, Vol. 180, No. 22
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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