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Journal of Bacteriology, December 1998, p. 6077-6081, Vol. 180, No. 23
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Transcriptional Analysis of the Staphylococcus aureus Penicillin Binding Protein 2 Gene

Mariana G. Pinho,1,2 Herminia de Lencastre,1,2,3 and Alexander Tomasz1,*

Laboratory of Microbiology, The Rockefeller University, New York, New York 10021,1 and Molecular Genetics Unit, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras,2 and Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Monte da Caparica,3 Portugal

Received 14 August 1998/Accepted 24 September 1998

Sequencing of the vicinity of the staphylococcal pbp2 gene and transcriptional analysis by primer extension and promoter fusions were used to show that pbp2 is part of an operon that also includes a gene with high homology to prfA of Bacillus subtilis. Two distinct promoters were identified directing transcription of pbp2 either alone or together with prfA. It was recently reported that transposon inactivation of pbp2 causes a reduction in methicillin resistance, but complementation experiments were not fully successful. We now show that introduction of the intact pbp2 gene with its two newly identified promoters into the chromosome of the transposon mutant resulted in the full recovery of high-level methicillin resistance.

* Corresponding author. Mailing address: Laboratory of Microbiology, The Rockefeller University, 1230 York Avenue, New York, NY 10021. Phone: (212) 327-8278. Fax: (212) 327-8688. E-mail: tomasz{at}rockvax.rockefeller.edu.

Journal of Bacteriology, December 1998, p. 6077-6081, Vol. 180, No. 23
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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