This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Johansson, J.
Right arrow Articles by Uhlin, B. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Johansson, J.
Right arrow Articles by Uhlin, B. E.

 Previous Article  |  Next Article 

Journal of Bacteriology, December 1998, p. 6117-6125, Vol. 180, No. 23
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

H-NS and StpA Proteins Stimulate Expression of the Maltose Regulon in Escherichia coli

Jörgen Johansson,1 Björn Dagberg,2 Evelyne Richet,3 and Bernt Eric Uhlin1,*

Department of Microbiology1 and Department of Physiology,2 Umeå University, S-90187 Umeå, Sweden, and Unité de Génétique Moléculaire, Institut Pasteur, 75724 Paris Cedex 15, France3

Received 11 March 1998/Accepted 4 September 1998

The nucleoid-associated protein H-NS is a major component of the chromosome-protein complex, and it is known to influence the regulation of many genes in Escherichia coli. Its role in gene regulation is manifested by the increased expression of several gene products in hns mutant strains. Here we report findings showing that H-NS and the largely homologous protein StpA play a positive role in the expression of genes in the maltose regulon. In studies with hns mutant strains and derivatives also deficient in the stpA gene, we found that expression of the LamB porin was decreased. Our results showed that the amounts of both LamB protein and lamB mRNA were greatly reduced in hns and hns-stpA mutant strains. The same results were obtained when we monitored the amount of transcription from the malEFG operon. The lamB gene is situated in the malKlamBmalM operon, which forms a divergent operon complex together with the malEFG operon. The activation of these genes depends on the action of the maltose regulon activator MalT and the global activator cyclic AMP receptor protein. Using a malT-lacZ translational fusion and antiserum raised against MalT to measure the expression of MalT, we detected reduced MalT expression in hns and hns-stpA mutant strains in comparison with the wild-type strain. Our results suggest that the H-NS and StpA proteins stimulate MalT translation and hence play a positive role in the control of the maltose regulon.

* Corresponding author. Mailing address: Department of Microbiology, Umeå University, S-90187 Umeå, Sweden. Phone: 46-90-7856731. Fax: 46-90-772630. E-mail: bernt.eric.uhlin{at}micro.umu.se.

Journal of Bacteriology, December 1998, p. 6117-6125, Vol. 180, No. 23
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Werlang, I. C. R., Schneider, C. Z., Mendonca, J. D., Palma, M. S., Basso, L. A., Santos, D. S. (2009). Identification of Rv3852 as a nucleoid-associated protein in Mycobacterium tuberculosis. Microbiology 155: 2652-2663 [Abstract] [Full Text]  
  • Lengsfeld, C., Schonert, S., Dippel, R., Boos, W. (2009). Glucose- and Glucokinase-Controlled mal Gene Expression in Escherichia coli. J. Bacteriol. 191: 701-712 [Abstract] [Full Text]  
  • Silva, A. J., Sultan, S. Z., Liang, W., Benitez, J. A. (2008). Role of the Histone-Like Nucleoid Structuring Protein in the Regulation of rpoS and RpoS-Dependent Genes in Vibrio cholerae. J. Bacteriol. 190: 7335-7345 [Abstract] [Full Text]  
  • Hernandez-Lucas, I., Gallego-Hernandez, A. L., Encarnacion, S., Fernandez-Mora, M., Martinez-Batallar, A. G., Salgado, H., Oropeza, R., Calva, E. (2008). The LysR-Type Transcriptional Regulator LeuO Controls Expression of Several Genes in Salmonella enterica Serovar Typhi. J. Bacteriol. 190: 1658-1670 [Abstract] [Full Text]  
  • Corbett, D., Bennett, H. J., Askar, H., Green, J., Roberts, I. S. (2007). SlyA and H-NS Regulate Transcription of the Escherichia coli K5 Capsule Gene Cluster, and Expression of slyA in Escherichia coli Is Temperature-dependent, Positively Autoregulated, and Independent of H-NS. J. Biol. Chem. 282: 33326-33335 [Abstract] [Full Text]  
  • Perez-Gutierrez, C., Llompart, C. M., Skurnik, M., Bengoechea, J. A. (2007). Expression of the Yersinia enterocolitica pYV-Encoded Type III Secretion System Is Modulated by Lipopolysaccharide O-Antigen Status. Infect. Immun. 75: 1512-1516 [Abstract] [Full Text]  
  • Yang, J., Baldi, D. L., Tauschek, M., Strugnell, R. A., Robins-Browne, R. M. (2007). Transcriptional Regulation of the yghJ-pppA-yghG-gspCDEFGHIJKLM Cluster, Encoding the Type II Secretion Pathway in Enterotoxigenic Escherichia coli. J. Bacteriol. 189: 142-150 [Abstract] [Full Text]  
  • Wolf, T., Janzen, W., Blum, C., Schnetz, K. (2006). Differential Dependence of StpA on H-NS in Autoregulation of stpA and in Regulation of bgl.. J. Bacteriol. 188: 6728-6738 [Abstract] [Full Text]  
  • Balsalobre, C., Johansson, J., Uhlin, B. E. (2006). Cyclic AMP-Dependent Osmoregulation of crp Gene Expression in Escherichia coli.. J. Bacteriol. 188: 5935-5944 [Abstract] [Full Text]  
  • Oshima, T., Ishikawa, S., Kurokawa, K., Aiba, H., Ogasawara, N. (2006). Escherichia coli Histone-Like Protein H-NS Preferentially Binds to Horizontally Acquired DNA in Association with RNA Polymerase. DNA Res 13: 141-153 [Abstract] [Full Text]  
  • Rodriguez, S., Nieto, J. M., Madrid, C., Juarez, A. (2005). Functional Replacement of the Oligomerization Domain of H-NS by the Hha Protein of Escherichia coli. J. Bacteriol. 187: 5452-5459 [Abstract] [Full Text]  
  • Rouquette, C., Serre, M.-C., Lane, D. (2004). Protective Role for H-NS Protein in IS1 Transposition. J. Bacteriol. 186: 2091-2098 [Abstract] [Full Text]  
  • Wyborn, N. R., Stapleton, M. R., Norte, V. A., Roberts, R. E., Grafton, J., Green, J. (2004). Regulation of Escherichia coli Hemolysin E Expression by H-NS and Salmonella SlyA. J. Bacteriol. 186: 1620-1628 [Abstract] [Full Text]  
  • Sand, O., Gingras, M., Beck, N., Hall, C., Trun, N. (2003). Phenotypic characterization of overexpression or deletion of the Escherichia coli crcA, cspE and crcB genes. Microbiology 149: 2107-2117 [Abstract] [Full Text]  
  • Madrid, C., Nieto, J. M., Paytubi, S., Falconi, M., Gualerzi, C. O., Juarez, A. (2002). Temperature- and H-NS-Dependent Regulation of a Plasmid-Encoded Virulence Operon Expressing Escherichia coli Hemolysin. J. Bacteriol. 184: 5058-5066 [Abstract] [Full Text]  
  • Krin, E., Sismeiro, O., Danchin, A., Bertin, P. N. (2002). The regulation of Enzyme IIAGlc expression controls adenylate cyclase activity in Escherichia coli. Microbiology 148: 1553-1559 [Abstract] [Full Text]  
  • Nieto, J. M., Madrid, C., Miquelay, E., Parra, J. L., Rodriguez, S., Juarez, A. (2002). Evidence for Direct Protein-Protein Interaction between Members of the Enterobacterial Hha/YmoA and H-NS Families of Proteins. J. Bacteriol. 184: 629-635 [Abstract] [Full Text]  
  • KUMAR, S. S., SANKARAN, K., HAIGH, R., WILLIAMS, P. H., BALAKRISHNAN, A. (2001). Cytopathic effects of outer-membrane preparations of enteropathogenic Escherichia coli and co-expression of maltoporin with secretory virulence factor, EspB. J Med Microbiol 50: 602-612 [Abstract] [Full Text]  
  • Johansson, J., Eriksson, S., Sondén, B., Wai, S. N., Uhlin, B. E. (2001). Heteromeric Interactions among Nucleoid-Associated Bacterial Proteins: Localization of StpA-Stabilizing Regions in H-NS of Escherichia coli. J. Bacteriol. 183: 2343-2347 [Abstract] [Full Text]  
  • Sardesai, A. A., Gowrishankar, J. (2001). trans-Acting Mutations in Loci Other than kdpDE That Affect kdp Operon Regulation in Escherichia coli: Effects of Cytoplasmic Thiol Oxidation Status and Nucleoid Protein H-NS on kdp Expression. J. Bacteriol. 183: 86-93 [Abstract] [Full Text]  
  • Westermark, M., Oscarsson, J., Mizunoe, Y., Urbonaviciene, J., Uhlin, B. E. (2000). Silencing and Activation of ClyA Cytotoxin Expression in Escherichia coli. J. Bacteriol. 182: 6347-6357 [Abstract] [Full Text]  
  • Schreiber, V., Richet, E. (1999). Self-association of the Escherichia coli Transcription Activator MalT in the Presence of Maltotriose and ATP. J. Biol. Chem. 274: 33220-33226 [Abstract] [Full Text]  
  • Johansson, J., Uhlin, B. E. (1999). Differential protease-mediated turnover of H-NS and StpA revealed by a mutation altering protein stability and stationary-phase survival of Escherichia coli. Proc. Natl. Acad. Sci. USA 96: 10776-10781 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»