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Journal of Bacteriology, December 1998, p. 6140-6147, Vol. 180, No. 23
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

uspB, a New sigma S-Regulated Gene in Escherichia coli Which Is Required for Stationary-Phase Resistance to Ethanol

Anne Farewell,* Kristian Kvint, and Thomas Nyström

Department of Microbiology, Lund University, Lund, Sweden

Received 23 March 1998/Accepted 30 September 1998

The open reading frame immediately upstream of uspA is demonstrated to encode a 14-kDa protein which we named UspB (universal stress protein B) because of its general responsiveness to different starvation and stress conditions. UspB is predicted to be an integral membrane protein with at least one and perhaps two membrane-spanning domains. Overexpression of UspB causes cell death in stationary phase, whereas mutants of uspB are sensitive to exposure to ethanol but not heat in stationary phase. In contrast to uspA, stationary-phase induction of uspB requires the sigma factor sigma S. The expression of uspB is modulated by H-NS, consistent with the role of H-NS in altering sigma S levels. Our results demonstrate that a gene of the RpoS regulon is involved in the development of stationary-phase resistance to ethanol, in addition to the regulon's previously known role in thermotolerance, osmotolerance, and oxidative stress resistance.

* Corresponding author. Present address: CMB-Microbiology, Göteborg University, Lundberg Laboratory, Box 462, 405 30 Göteborg, Sweden. Phone: 46 31 773 2567. Fax: 46 31 773 2599. E-mail: anne.farewell{at}gmm.gu.se.

Journal of Bacteriology, December 1998, p. 6140-6147, Vol. 180, No. 23
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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