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Journal of Bacteriology, December 1998, p. 6306-6315, Vol. 180, No. 23
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

RecA-Independent Pathways of Lambdoid Prophage Induction in Escherichia coli

Dmitry V. Rozanov,1 Richard D'Ari,2 and Sergey P. Sineoky1,*

State Scientific Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow 113545, Russia,1 and Institut Jacques Monod (Centre National de la Recherche Scientifique, Université Paris 7), 75251 Paris Cedex 05, France2

Received 21 July 1997/Accepted 1 October 1998

Two Escherichia coli genes, expressed from multicopy plasmids, are shown to cause partial induction of prophage lambda  in recA mutant lysogens. One is rcsA, which specifies a positive transcriptional regulator of the cps genes, which are involved in capsular polysaccharide synthesis. The other is dsrA, which specifies an 85-nucleotide RNA that relieves repression of the rcsA gene by histone-like protein H-NS. Genetic contexts known to increase Cps expression also cause RecA-independent lambda  induction: the rcsC137 mutation, which causes constitutive Cps expression, and the lon and rcsA3 mutations, which stabilize RcsA. Lambdoid phages 21, phi 80, and 434 are also induced by RcsA and DsrA overexpression in recA lysogens. Excess lambda  cI repressor specifically blocks lambda  induction, suggesting that induction involves repressor inactivation rather than repressor bypass. RcsA-mediated induction requires RcsB, the known effector of the cps operon, whereas DsrA-mediated induction is RcsB independent in stationary phase, pointing to the existence of yet another RecA-independent pathway of prophage induction.

* Corresponding author. Mailing address: State Scientific Research Institute of Genetics and Selection of Industrial Microorganisms, I Dorozhny Proezd 1, Moscow 113545, Russia. Phone: (7 095) 315 12 10. Fax: (7 095) 315 05 01. E-mail: vkpm{at}vnigen.msk.su.

Journal of Bacteriology, December 1998, p. 6306-6315, Vol. 180, No. 23
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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