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Journal of Bacteriology, December 1998, p. 6400-6403, Vol. 180, No. 23
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Role of C-Terminal Domains in Surface Attachment of the Fructosyltransferase of Streptococcus salivarius ATCC 25975

Catherine Rathsam and Nicholas A. Jacques*

Institute of Dental Research, Surry Hills, New South Wales 2010, Australia

Received 18 May 1998/Accepted 16 September 1998

The cell-associated beta -D-fructosyltransferase of Streptococcus salivarius, which is devoid of the cell wall anchoring motif, LPXTG, is released on exposure to its substrate, sucrose. Deletions within the C terminus of the enzyme implicated both the hydrophobic and the proline-glycine-serine-threonine-rich wall-associated domain in stabilizing the enzyme on the cell surface.


* Corresponding author. Mailing address: Institute of Dental Research, 2 Chalmers St., Surry Hills, NSW 2010, Australia. Phone: 61-2-9293-3353. Fax: 61-2-9293-3368. E-mail: nickj{at}dentistry.usyd.edu.au.


Journal of Bacteriology, December 1998, p. 6400-6403, Vol. 180, No. 23
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.



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