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Journal of Bacteriology, December 1998, p. 6404-6407, Vol. 180, No. 23
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

PRS5, the Fifth Member of the Phosphoribosyl Pyrophosphate Synthetase Gene Family in Saccharomyces cerevisiae, Is Essential for Cell Viability in the Absence of either PRS1 or PRS3

Yolanda Hernando, Adrian Parr, and Michael Schweizer*

Genetics and Microbiology Department, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, United Kingdom

Received 20 July 1998/Accepted 23 September 1998

In Saccharomyces cerevisiae, an open reading frame, YOL061w, encodes a polypeptide with sequence similarity to the four known 5-phosphoribosyl-1(alpha )-pyrophosphate synthetase (PRS) genes since it contains a divalent cation binding site and a phosphoribosyl pyrophosphate binding site. We regard YOL061w as the fifth member of the PRS gene family, PRS5. Loss of Prs5p has a significant impact on PRS enzyme activity, causing it to be reduced by 84%. On the other hand, Delta prs5 strains are not affected in growth or in the size of their nucleotide pools. However, simultaneous deletion of PRS1 and PRS5 or PRS3 and PRS5 rendered the strains inviable, which implies that PRS5 plays an important role in the maintenance of PRS function in S. cerevisiae.

* Corresponding author. Mailing address: Genetics & Microbiology Dept., Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, United Kingdom. Phone: 44 1603 255250. Fax: 44 1603 458414. E-mail: Michael.Schweizer{at}BBSRC.AC.UK.

Journal of Bacteriology, December 1998, p. 6404-6407, Vol. 180, No. 23
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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