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Journal of Bacteriology, December 1998, p. 6544-6550, Vol. 180, No. 24
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Inhibition of Development of Myxococcus xanthus by Eukaryotic Protein Kinase Inhibitors

Ritu Jain and Sumiko Inouye*

Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854

Received 23 March 1998/Accepted 14 September 1998

Myxococcus xanthus is a social bacterium that lives in the soil and undergoes spectacular development to form multicellular fruiting bodies. It contains a large family of eukaryote-like serine/threonine protein kinases. We found that a number of inhibitors for eukaryotic protein serine, threonine, and tyrosine kinases could inhibit the development and sporulation of M. xanthus to various degrees. These results suggest that serine/threonine and tyrosine phosphorylation may be involved in development of M. xanthus. None of the inhibitors tested had any effect on vegetative growth of M. xanthus. Most of them seemed to act during the early stages of development. However, the expression of a very early development-specific gene, Omega 4521, was not significantly affected by the inhibitors. The patterns of protein phosphorylation during development were also not significantly altered by the inhibitors, suggesting that the targets of the inhibitors are minor or unstable phosphoproteins but play key roles in fruiting-body formation in M. xanthus.

* Corresponding author. Mailing address: Department of Biochemistry, UMDNJ-RWJMS, 675 Hoes Lane, Piscataway, NJ 08854. Phone: (732) 235-4161. Fax: (732) 235-4559. E-mail: sinovy{at}waksman.rutgers.edu.

Journal of Bacteriology, December 1998, p. 6544-6550, Vol. 180, No. 24
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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