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Journal of Bacteriology, December 1998, p. 6764-6768, Vol. 180, No. 24
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Novel Serine/Threonine Protein Kinase Homologue of Pseudomonas aeruginosa Is Specifically Inducible within the Host Infection Site and Is Required for Full Virulence in Neutropenic Mice

Jingyi Wang,1 Caihe Li,1 Hongjiang Yang,1 Arcady Mushegian,2 and Shouguang Jin1,*

Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205,1 and Axys Pharmaceuticals, Inc., La Jolla, California 920372

Received 21 July 1998/Accepted 8 October 1998

A genetic locus of Pseudomonas aeruginosa was identified that is highly and specifically inducible during infection of neutropenic mice. This locus, ppkA, encodes a protein that is highly homologous to eukaryote-type serine/threonine protein kinases. A ppkA null mutant strain shows reduced virulence in neutropenic mice compared to the wild type. Overexpression of the PpkA protein greatly inhibited the growth of Escherichia coli or P. aeruginosa. However, a single amino acid change at the catalytic site of the kinase domain eliminated the toxic effect of PpkA on bacterial cells, suggesting that the kinase domain of PpkA is functional within bacterial cells.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Mail Slot 511, 4301 W. Markham, Little Rock, AR 72205. Phone: (501) 296-1396. Fax: (501) 686-5359. E-mail: JINSHOUGUANG{at}EXCHANGE.UAMS.EDU.

Journal of Bacteriology, December 1998, p. 6764-6768, Vol. 180, No. 24
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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